The front-end logistics of DNA barcoding: challenges and prospects
The front-end logistics of DNA barcoding: challenges and prospects
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DOI：
10.1111/j.1755-0998.2009.02629.x
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发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Hebert, Paul D. N.
中科院分区：
文献类型：
--
作者：
Borisenko, Alex V.;Sones, Jayme E.;Hebert, Paul D. N.
建立全球DNA条形码库需要实验室前样本处理的高效物流以及与分子协议的无缝对接。如果处理不当，样本汇总工作可能成为分析链中的最大瓶颈。加拿大DNA条形码中心在开发一个便利高通量DNA条形码的收集管理系统方面有三年的经验，这使它能够认识和解决最常见的后勤障碍。在更大范围内应对这些挑战将是为国际DNA条形码工作建立一个坚实的收集基础的重要一步。
Building a global library of DNA barcodes will require efficient logistics of pre-laboratory specimen processing and seamless interfacing with molecular protocols. If not addressed properly, the task of aggregating specimens may become the biggest bottleneck in the analytical chain. Three years of experience in developing a collection management system to facilitate high-throughput DNA barcoding have allowed the Canadian Centre for DNA Barcoding to recognize and resolve the most common logistical obstacles. Dealing with these challenges on a larger scale will be an important step towards building a solid collection-based foundation for the international DNA barcoding effort.