SSEA-1 is a specific marker for the spinal sensory neuron lineage in the quail embryo and in neural crest cell cultures.
SSEA-1 is a specific marker for the spinal sensory neuron lineage in the quail embryo and in neural crest cell cultures.
复制标题
SSEA-1 是鹌鹑胚胎和神经嵴细胞培养物中脊髓感觉神经元谱系的特异性标记。
DOI：
10.1016/0012-1606(89)90108-5
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发表时间：
1989
影响因子：
2.7
通讯作者：
Sieber-Blum,M
中科院分区：
文献类型：
--
作者：
Sieber-Blum,M
对初级感觉神经元发育早期阶段的研究仅限于从感觉神经节获得的细胞。由于缺乏早期的、谱系特异性的感觉神经母细胞标记物，因此不可能使用神经嵴作为前体细胞的来源，而神经嵴是产生所有脊髓和一些颅初级感觉神经元的地方。在本研究中，我们发现在鹌鹑胚胎的神经嵴衍生物中，阶段特异性胚胎抗原-1 （SSEA-1）通过发育中的感觉神经母细胞特异性表达。用抗ssea -1和AC4单克隆抗体在体内和神经嵴细胞培养中表征感觉神经元的发育。在大鼠和小鼠中，两种抗体识别相同的碳水化合物序列[半乳糖β1-4（聚焦α -1 - 3） n -乙酰氨基葡萄糖]，这是SSEA-1的特征。在鹌鹑胚胎中，这个表位是一个具有几个吸引人的特征的标记。在神经嵴衍生物中，它对感觉谱系是特异性的，并在所有脊柱轴水平的所有可检测到的感觉神经母细胞中表达。此外，碳水化合物序列出现较早，并在整个发育过程中持续存在。我们还研究了SSEA-1在神经嵴细胞培养中的表达，其中观察了两群感觉神经母细胞。一个居群在外植入培养物之前或之后不久分化；这些细胞并没有从神经管中迁移出来。第二种人口出现在更古老的文化中。在未迁移的SSEA-1+细胞3天后，它们形成迁移神经嵴细胞的前缘，成为SSEA-1+。双染色实验显示两种群在形态学、神经丝表达和神经递质含量方面无明显差异。
Investigation of the early phases of the development of primary sensory neurons has been limited to cells obtained from sensory ganglia. Due to the lack of an early, lineage-specific marker for sensory neuroblasts, it has not been possible to use the neural crest, which gives rise to all spinal and some cranial primary sensory neurons, as a source of precursor cells. In the present study, we show that in neural crest derivatives of the quail embryo, the stage-specific embryonic antigen-1 (SSEA-1) is expressed specifically by developing sensory neuroblasts. The monoclonal antibodies anti-SSEA-1 and AC4 were used to characterize sensory neuron developmentin vivoand in neural crest cell cultures. In the rat and mouse, both antibodies recognize the same carbohydrate sequence [galactose β1–4(fucose α1–3)N-acetylglucosamine] which characterizes SSEA-1. In the quail embryo, this epitope is a marker with several attractive characteristics. Among neural crest derivatives, it is specific for the sensory lineage and is expressed by all detectable sensory neuroblasts at all spinal axial levels. In addition, the carbohydrate sequence appears early and persists throughout development. Expression of SSEA-1 was also studied in neural crest cell cultures, in which two populations of sensory neuroblasts were observed. One population differentiated before or shortly after explantation into culture; these cells did not emigrate from the neural tube. A second population appeared in older cultures. Forming the leading edge of the emigrating neural crest cells, they became SSEA-1+3 days after the nonmigrating SSEA-1+cells. Double staining experiments revealed no obvious differences between the two populations with regard to morphology, neurofilament expression, and neurotransmitter content.