REQUIREMENT FOR RAS PROTO-ONCOGENE FUNCTION DURING SERUM-STIMULATED GROWTH OF NIH-3T3-CELLS
REQUIREMENT FOR RAS PROTO-ONCOGENE FUNCTION DURING SERUM-STIMULATED GROWTH OF NIH-3T3-CELLS
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DOI：
10.1038/313241a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
STACEY, DW
中科院分区：
文献类型：
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作者：
MULCAHY, LS;SMITH, MR;STACEY, DW
人类肿瘤通常含有在正常组织中未发现的DNA序列，其能够转化培养的NIH 3T3细胞。在某些肿瘤中，负责这种转化的基因属于cellularras基因家族1，2。特定类型的突变负责将细胞原癌基因转化为能够诱导转化的arasoncogene 3 - 5。在一项关于cellularras基因功能的研究中，将其蛋白质产物（在细菌细胞中产生）显微注射到NIH 3T3细胞中;受体细胞在形态上发生了转化，并在没有添加血清的情况下被诱导启动DNA合成6，但只有当cellularras蛋白质以比转化基因蛋白质所需浓度高得多的浓度注射时才能如此6，7。为了进一步分析cellarras基因的功能，我们将抗cellarras蛋白的单克隆抗体注射到NIH 3T3细胞中。本文报道用血清诱导分裂的NIH 3T3细胞，在显微注射抗ras抗体后，不能进入细胞周期的S期，表明NIH 3T3细胞S期的起始需要ras原癌基因的蛋白产物。
Human tumours often contain DNA sequences not found in normal tissues which are able to transform cultured NIH 3T3 cells. In some tumours the gene responsible for this transformation belongs to the cellularrasgene family1,2. A specific type of mutation is responsible for converting the cellular proto-oncogene into arasoncogene capable of inducing transformation3–5. In a study of the function of a cellularrasgene, its protein product (produced in a bacterial cell) was microinjected into NIH 3T3 cells; the recipient cells became morphologically transformed and were induced to initiate DNA synthesis in the absence of added serum6, but only when cellularrasprotein was injected at much higher concentrations than required with protein of the transformingrasgene6,7. To further analyse the function of the cellularrasgene, we have now injected monoclonal antibodies againstrasproteins into NIH 3T3 cells. We report here that NIH 3T3 cells induced to divide by adding serum to the culture medium are unable to enter the S phase of the cell cycle after microinjection of anti-rasantibody, showing that the protein product of therasproto-oncogene is required for initiation of the S-phase in NIH 3T3 cells.