Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli
Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli
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DOI：
10.1016/j.vaccine.2008.02.023
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发表时间：
2008-04-07
期刊：
影响因子：
5.5
通讯作者：
Manganelli, Riccardo
中科院分区：
文献类型：
--
作者：
Brun, Paola;Zumbo, Antonella;Manganelli, Riccardo
天然侵入性细菌已成功地用于粘膜递送针对细菌、病毒和肿瘤抗原的DNA疫苗。最近，一个替代的传递系统的基础上的非致病性的大肠杆菌的基因修饰的突变体，并成功地用于提供治疗基因和免疫原性蛋白的上皮细胞在vivo.In这项工作中，我们使用这些重组侵入性细菌提供DNA疫苗对两个结核分枝杆菌蛋白（FbpA，和HtpX）后鼻内给药。两种DNA疫苗都能够诱导抗原特异性T细胞应答。此外，用携带编码HtpX的DNA疫苗的重组细菌免疫小鼠，对M.结核(c)2008爱思唯尔有限公司保留所有权利。
Naturally invasive bacteria have been successfully used for mucosal delivery of DNA vaccines against bacteria[, viral and tumour antigens. Recently, an alternative delivery system based on a genetically modified mutant of the non-pathogenic commensal bacterium Escherichia coli, was developed and successfully used to deliver therapeutic genes and immunogenic proteins to epithelial cells in vivo.In this work, we used these recombinant invasive bacteria to deliver DNA vaccines against two Mycobacterium tuberculosis proteins (FbpA, and HtpX) following intranasal administration. Both DNA vaccines were able to induce an antigen-specific T-cell response. Moreover, mice immunized with the recombinant bacteria carrying the DNA vaccine encoding HtpX, were significatively protected from challenge with M. tuberculosis. (c) 2008 Elsevier Ltd. All rights reserved.