PLUNC (palate, lung and nasal epithelial clone) proteins in human nasal lavage fluid
PLUNC (palate, lung and nasal epithelial clone) proteins in human nasal lavage fluid
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DOI：
10.1042/bst0310810
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发表时间：
2003-08-01
影响因子：
3.9
通讯作者：
Lindahl, M
中科院分区：
文献类型：
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作者：
Ghafouri, B;Kihlström, E;Lindahl, M
plincc（腭、肺和鼻上皮克隆）是一个新发现的在上呼吸道表达的基因，被认为在宿主防御细菌中起重要作用。我们利用二维凝胶电泳（2-DE）和质谱技术在人鼻灌洗液（NLF）中鉴定出了两种形式的PLUNC蛋白，这两种形式的表观分子质量和等电点分别为24.8 kDa/pl 5.4和25.1 kDa/pl S.S.。值得注意的是，24.8 kDa/pl 5.4形式的PLUNC是健康受试者鼻灌洗液2-DE蛋白模式中的丰富蛋白。在暴露于活性环氧化学物质的吸烟者和工人的NLF中发现PLUNC水平降低，表明长期暴露于气道刺激物会损害上呼吸道中PLUNC的产生。我们还研究了NLF中脂多糖（LPS）结合蛋白的存在。发现5种蛋白被吸附在lps包被的表面上；根据2-DE模式匹配判断，其中两种蛋白质对应于两种PLUNC形式。相比之下，人类唾液中含有一组具有相似2-DE位点的lps结合蛋白（相同的pis，但表观分子质量略低，约为20 kDa）。这些结果表明，PLUNC可能是气道炎症的新标志物，可能在先天免疫反应中发挥作用，并且人类唾液中还含有lps结合蛋白家族的其他成员。
PLUNC (palate, lung and nasal epithelial clone) is a newly discovered gene that is expressed in the upper respiratory tract and is suggested to be of importance in host defence against bacteria. We have identified two forms of the PLUNC protein in human nasal lavage fluid (NLF) using two-dimensional gel electrophoresis (2-DE) and MS. The apparent molecular masses and isoelectric points of these forms are 24.8 kDa/pl 5.4 and 25.1 kDa/pl S.S. Notably, the 24.8 kDa/pl 5.4 form of PLUNC is an abundant protein in the 2-DE protein patterns of NLF from healthy subjects. Decreased levels of PLUNC were found in NLF from smokers and workers exposed to reactive epoxy chemicals, indicating that long-term exposure to airway irritants impairs the production of PLUNC in the upper respiratory tract. We have also investigated the presence of lipopolysaccharide (LPS)-binding proteins in NLF. Five proteins were found to adsorb to a LPS-coated surface; two of these proteins correspond to the two PLUNC forms, as judged by 2-DE pattern matching. For comparison, human saliva was found to contain a set of LPS-binding proteins with similar 2-DE spot positions (the same pis but somewhat lower apparent molecular masses of approximate to20 kDa). These results indicate that PLUNC may be a new marker of airway inflammation and may play a part in the innate immune response, and that human saliva contains yet other members of the family of LPS-binding proteins.