Differentiation of triticale cultivars through FISH karyotyping of their rye chromosomes
Differentiation of triticale cultivars through FISH karyotyping of their rye chromosomes
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DOI：
10.1139/gen-2012-0117
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发表时间：
2013-05-01
期刊：
影响因子：
3.1
通讯作者：
Poggio, Lidia
中科院分区：
文献类型：
--
作者：
Fradkin, Maia;Rosa Ferrari, Maria;Poggio, Lidia
本工作的目的是通过荧光原位杂交（FISH）分析黑麦染色体的细胞遗传学特征的小黑麦品种。在目前的工作中，我们研究了六个品种的小黑麦（'卡尤-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cucu-Cuc这些品种从国际玉米和小麦改良中心（CIMMYT）获得，并在UNRC进行了改良，用于新鲜牧草、干草和饲料谷物。黑麦（Secale cereale）（pSc74、pSc200、pSc250和pSc119.2）的高重复DNA序列的分布和组织利用FISH分析揭示了几个黑麦染色体的信号的特定定位，这使我们能够区分品种。聚类分析表明，用于产生这些杂交种的黑麦品种之间有很大的细胞遗传相似性。小黑麦品种之间的变异性的知识是必要的，提出未来的杂交育种计划。本研究对于鉴定商业种子和分析农艺性状与某些黑麦染色体或这些染色体中特定区域的存在之间的可能关联也将是有价值的。
The aim of this work was to cytogenetically characterize triticale cultivars through fluorescence in situ hybridization (FISH) analysis of their rye chromosomes. In the present work, we studied six cultivars of triticale ('Cayu-UNRC', 'Cume-UNRC', 'Genu-UNRC', 'Ninca-UNRC', 'Quine-UNRC', and 'Tizne-UNRC'), released by the Universidad Nacional de Rio Cuarto (UNRC), Cordoba, Argentina. The cultivars were obtained from the International Center for the Improvement of Maize and Wheat (CIMMYT) and improved for fresh forage, haymaking, and feed grain at UNRC. The distribution and organization of highly repetitive DNA sequences of Secale cereale (pSc74, pSc200, pSc250, and pSc119.2) using FISH analyses revealed a specific localization of the signals for several rye chromosomes, which allowed us to distinguish the cultivars. Cluster analysis showed a great cytogenetic similarity among the rye cultivars used to originate these hybrids. The knowledge of the variability among triticale cultivars is necessary to propose future crosses in breeding programs. This study will also be valuable to identify commercial seeds and to analyze the possible association between agronomic characters and the presence of certain rye chromosomes or specific regions in these chromosomes.