Antioxidant Capacity of Nitrogen and Sulfur Codoped Carbon Nanodots.
Antioxidant Capacity of Nitrogen and Sulfur Codoped Carbon Nanodots.
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DOI：
10.1021/acsanm.8b00404
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发表时间：
2018-05
影响因子：
5.9
通讯作者：
Wendi Zhang;J. Chavez;Z. Zeng;B. Bloom;Alex T. Sheardy;Zuowei Ji;Ziyu Yin;D. Waldeck;Z. Jia-Z.-J
中科院分区：
文献类型：
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作者：
Wendi Zhang;J. Chavez;Z. Zeng;B. Bloom;Alex T. Sheardy;Zuowei Ji;Ziyu Yin;D. Waldeck;Z. Jia-Z.-J
碳纳米点（CND）已显示出在细胞水平上具有抗氧化活性的潜力。本文以α-硫辛酸、柠檬酸和尿素为前体分子，采用水热法制备了氮硫共掺杂的荧光纳米碳纳米管。本文采用紫外-可见吸收光谱法、DPPH·电化学法和光泽精化学发光法研究了它们的抗氧化活性。光泽精-CL测定检测超氧阴离子自由基，即，通过黄嘌呤/黄嘌呤氧化酶（XO）反应产生的活性氧（ROS）。电化学推导的未反应的氮为中心的DPPH·和CND浓度之间的关系与从UV-vis测量得到的结果一致。提出了N，S共掺杂CNDs的ROS抗氧化反应的反应途径。这些发现应有助于N，S-共掺杂的CNDs在生物医学应用中的实际用途的发展。
Carbon nanodots (CNDs) have shown potential for antioxidative activity at the cellular level. Here we applied a facile hydrothermal method to prepare fluorescent nitrogen and sulfur (N,S-)codoped CNDs using α-lipoic acid, citric acid, and urea as precursor molecules. This work describes a comprehensive study for exploring their antioxidation activity using UV-vis absorption and electrochemistry measurements of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), as well as a lucigenin chemiluminescence (lucigenin-CL) assay. The lucigenin-CL assay detects superoxide anion radicals, i.e., reactive oxygen species (ROS) produced through the xanthine/xanthine oxidase (XO) reaction. The electrochemically derived relationship between the unreacted nitrogen-centered DPPH• and CND concentrations agrees with that obtained from UV-vis measurements. A reaction pathway for the ROS antioxidative reaction of N,S-codoped CNDs is proposed. These findings should aid in the development of N,S-codoped CNDs for practical use in biomedical applications.