Probing the cytotoxicity of CdSe quantum dots with surface modification
Probing the cytotoxicity of CdSe quantum dots with surface modification
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DOI：
10.1016/j.matlet.2006.07.105
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发表时间：
2007-04-01
影响因子：
3
通讯作者：
Yang, Xiangliang
中科院分区：
文献类型：
--
作者：
Guo, Guoning;Liu, Wei;Yang, Xiangliang
该论文首次报道了表面修饰的 CdSe 量子点（QD）的细胞毒性。将 CdSe QD 掺入聚 (D, L) 丙交酯 (PLA) 纳米颗粒中，然后分别用 Fluronic (R) 68 (F-68)、十六烷基三甲基溴化铵 (CTAB) 和十二烷基硫酸钠 (SDS) 进行表面修饰。采用纳米沉淀法制备了三种不同粒径和 zeta 电位的表面修饰 CdSe 量子点。通过 MTT 活力测定在 HepG2 细胞模型中评估表面修饰的 CdSe QD 的细胞毒性。结果表明，表面修饰的CdSe量子点的体外细胞毒性取决于表面性质。 F-68 和 SDS 的表面修饰可以减轻 CdSe QD 的细胞毒性，而 CTAB 的表面修饰则显示出显着的细胞损伤。将表面经F-68修饰的CdSe量子点注射入小鼠体内，获得内脏荧光图像。结果表明，F-68 表面修饰的 CdSe 量子点具有较低的细胞毒性，在生物标记和成像应用方面具有良好的潜力。 (c) 2006 Elsevier B.V. 保留所有权利。
The cytotoxicity of CdSe quantum dots (QDs) with surface modification was reported first in the paper. CdSe QDs were incorporated into poly (D, L) lactide (PLA) nanoparticles and then surface modified with Fluronic (R) 68 (F-68), cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulfate (SDS), respectively. Three different particle sizes and zeta potential of the surface modified CdSe QDs were produced using a nano-precipitation method. The cytotoxicity of the surface modified CdSe QDs was evaluated in HepG2 cell model with MTT viability assay. The results showed that the cytotoxicity of the surface modified CdSe QDs in vitro was dependent on the surface properties. Surface modification with F-68 and SDS could lessen the cytotoxicity of CdSe QDs, while surface modification with CTAB showed significant cell damage. CdSe QDs surface modified with F-68 were injected into mice and the fluorescence images in viscus were obtained. The results suggested that CdSe QDs surface modified with F-68 have low cytotoxicity and good potential for biological labeling and imaging applications. (c) 2006 Elsevier B.V. All rights reserved.