Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells.
Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells.
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敲低长链非编码RNA CCAT2可抑制神经胶质瘤细胞的增殖和迁移
DOI：
10.18632/oncotarget.13242
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发表时间：
2016-12-06
期刊：
影响因子：
--
通讯作者：
Wu L
中科院分区：
文献类型：
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作者：
Guo H;Hu G;Yang Q;Zhang P;Kuang W;Zhu X;Wu L
结肠癌相关转录物2（CCAT 2）的长非编码RNA通常在许多癌症中进行研究。然而，其在胶质瘤生物学中的表达和生物学功能知之甚少。在本研究中，我们使用定量实时PCR（qRT-PCR）来确定CCAT 2在胶质瘤组织中的表达。我们发现CCAT 2在胶质瘤组织中表达上调，并与晚期肿瘤（III/IV期）显著相关。体内外功能实验表明，CCAT 2基因的敲低可抑制胶质瘤细胞的增殖、细胞周期进程和迁移。进一步的分析表明CCAT 2基因敲低可能通过抑制Wnt/β-catenin信号通路的活性而影响胶质瘤细胞表型。因此，我们的研究提供了证据表明，CCAT 2可能作为一个潜在的生物标志物的胶质瘤。
Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, we used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. We found that expression of CCAT2 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Functional assays in vitro and in vivo demonstrated that knockdown of CCAT2 could inhibit proliferation, cell cycle progression and migration of glioma cells. Further analysis indicated the effect of CCAT2 knockdown on glioma cell phenotype through inhibiting Wnt/β-catenin signal pathway activity. Thus, our study provides evidence that CCAT2 may function as a potential biomarker for glioma.