Experimental simulation of macromolecules in trehalose aqueous solutions: A photon correlation spectroscopy study
Experimental simulation of macromolecules in trehalose aqueous solutions: A photon correlation spectroscopy study
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海藻糖水溶液中大分子的实验模拟：光子相关光谱研究
DOI：
10.1063/1.480250
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
V. Villari
中科院分区：
文献类型：
--
作者：
S. Magazù;G. Maisano;P. Migliardo;V. Villari
海藻糖对生物膜防止冷冻或脱水的保护作用一直是许多研究的主题，旨在了解一些低等生物在应激条件下合成海藻糖的原因。在这项工作中，我们报告了通过光子相关光谱法对聚（环氧乙烷）/海藻糖/水混合物进行的研究结果。该聚合物的化学结构比蛋白质及其在水中的螺旋构象更简单，为理解更复杂的蛋白质/海藻糖/水相互作用提供了一个有用的起点。为了区分不同的动力学，在水中、不同浓度和温度值下分别研究了海藻糖和 PEO；然后在不同糖量下研究了三元PEO/海藻糖/水系统。获得的发现支持“水替代”假说，表明发生了直接的聚合物-海藻糖相互作用。此外，海藻糖被证明会影响膨胀......
The protective effect of trehalose on biological membranes against freezing or dehydration has been the subject of many studies aimed to understand the reasons why some lower organisms, under stress conditions, synthesize trehalose. In this work we report the results of a study on Poly(Ethylene Oxide)/trehalose/water mixtures performed by Photon Correlation Spectroscopy. The chemical structure of the polymer, simpler than that of proteins and its helical conformation in water, constitute a useful starting point for understanding the more complex protein/trehalose/water interactions. In order to distinguish the different dynamics, trehalose and PEO have been studied separately in water, at different concentration and temperature values; then the ternary PEO/trehalose/water system has been investigated at different sugar amounts. The obtained findings support the “water-replacement” hypothesis, indicating that a direct polymer–trehalose interaction occurs. Furthermore, trehalose is shown to affect the swell...