RELEASE OF [3H]‐ACETYLCHOLINE FROM THE ISOLATED RETINA OF THE RAT BY POTASSIUM DEPOLARIZATION: DEPENDENCE ON HIGH AFFINITY CHOLINE UPTAKE
RELEASE OF [3H]‐ACETYLCHOLINE FROM THE ISOLATED RETINA OF THE RAT BY POTASSIUM DEPOLARIZATION: DEPENDENCE ON HIGH AFFINITY CHOLINE UPTAKE
复制标题
通过钾去极化从大鼠离体视网膜中释放 [3H]-乙酰胆碱：对高亲和力胆碱摄取的依赖性
DOI：
--
复制
发表时间：
1979
影响因子：
7.3
通讯作者：
M. Neal
中科院分区：
文献类型：
--
作者：
S. Massey;M. Neal
1研究了钾离子去极化对离体大鼠视网膜[3 H]-乙酰胆碱（[3 H]-ACh）释放的影响。2将视网膜暴露于含有KCl（50 mm）的培养基中，引起[3 H]-ACh流出量大幅增加，同时[3 H]-胆碱流出量仅小幅增加。在无钙培养基中，KCl诱发的[3 H]-ACh释放几乎被消除。[3]在无钠培养基或含半胆碱盐-3（HC-3）的培养基中孵育视网膜，这些被认为选择性抑制高亲和力胆碱转运系统的程序使[3 H]-胆碱的视网膜摄取减少约50%，[3 H]-ACh的合成减少约97%。[4]在无钠培养基或含HC-3的培养基中加载[3 H]-胆碱，随后在正常培养基中灌流的视网膜中，[3 H]-ACh的钾诱发释放几乎消失。[5]这表明，与神经系统的其他区域一样，钠依赖性、高亲和力的胆碱摄取系统在视网膜胆碱能神经末梢中很重要。
1 The effect of potassium depolarization on the release of [3H]‐acetylcholine ([3H]‐ACh) from the isolated retina of the rat was studied. 2 Exposure of retinae to medium containing KC1 (50 mm) evoked a large increase in the efflux of [3H]‐ACh with only a small concurrent increase in the efflux of [3H]‐choline. The KCl‐evoked release of [3H]‐ACh was almost abolished in calcium‐free medium. 3 Incubation of retinae with [3H]‐choline in sodium‐free medium, or medium containing hemi‐cholinium‐3 (HC‐3), procedures that are believed to inhibit selectively the high affinity choline transport system, reduced the retinal uptake of [3H]‐choline by approximately 50% and the synthesis of [3H]‐ACh by about 97%. 4 The potassium‐evoked release of [3H]‐ACh was almost abolished in retinae that had been loaded with [3H]‐choline in sodium‐free medium or medium containing HC‐3, and subsequently superfused in normal medium. 5 It is suggested that as in other areas of the nervous system, a sodium‐dependent, high affinity uptake system for choline is important in retinal cholinergic nerve terminals.