INVITRO ACTIVITY OF SOME GLUCOSINOLATES AND THEIR REACTION-PRODUCTS TOWARD A POPULATION OF THE NEMATODE HETERODERA-SCHACHTII
INVITRO ACTIVITY OF SOME GLUCOSINOLATES AND THEIR REACTION-PRODUCTS TOWARD A POPULATION OF THE NEMATODE HETERODERA-SCHACHTII
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DOI：
10.1021/jf00029a028
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发表时间：
1993-05-01
影响因子：
6.1
通讯作者：
PALMIERI, S
中科院分区：
文献类型：
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作者：
LAZZERI, L;TACCONI, R;PALMIERI, S
对某些芥子油苷及其黑芥子酶介导的酶水解产物对甜菜胞囊线虫 Heterodera schachtii 第二阶段幼虫的杀线虫作用进行了体外研究。所测试的芥子油苷是从被列为线虫宿主的一些十字花科植物的种子或植物器官中纯化的：甘蓝型油菜、独行菜、白菜、甘蓝、萝卜和白芥。在黑芥子酶不存在和存在的情况下，硫代葡萄糖苷以不同浓度溶解，然后测定它们对第二阶段幼体的杀线虫效果。所有测试的芥子油苷（即其天然形式）均未表现出杀线虫作用，而其中一些（黑芥子苷、葡糖苷、葡萄芥素、葡萄糖苷氢芥子素和从油菜籽中提取的整个芥子油苷基团）在 pH 7.0 下的酶水解产物（主要是异硫氰酸盐）表现出死亡率随浓度的变化而变化。产品和暴露时间。萝卜硫素和芥子宾的反应产物在测试浓度下没有表现出任何杀线虫活性。
An in vitro study was conducted of the nematocidal effect of some glucosinolates and the products of their myrosinase-mediated enzymatic hydrolysis on second-stage juveniles of the sugar beet cyst nematode Heterodera schachtii. The glucosinolates tested were purified from the seeds or from plant organs of some crucifers cited as hosts of the nematode: Brassica napus, Lepidium sativum, Brassica rapa, Brassica carinata, Raphanus sativus, and Sinapis alba. The glucosinolates were dissolved in various concentrations in both the absence and presence of the enzyme myrosinase, and their nematocidal effect in time on second-stage juveniles was then determined. All of the glucosinolates tested as such (i.e., in their native form) showed no nematocidal effect, whereas the products of the enzymatic hydrolysis at pH 7.0 (essentially isothiocyanate) of some of them (sinigrin, gluconapin, glucotropeolin, glucodehydroerucin, and the whole glucosinolate group extracted from rapeseed) demonstrated a mortality rate that varied as a function of both the concentration of product and the exposure time. The reaction products of glucoraphenin and sinalbin did not show any nematocidal activity at the concentrations tested.