Biomimetic Nanopatterns as Enabling Tools for Analysis and Control of Live Cells
Biomimetic Nanopatterns as Enabling Tools for Analysis and Control of Live Cells
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DOI：
10.1002/adma.201000468
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发表时间：
2010-11-02
影响因子：
29.4
通讯作者：
Levchenko, Andre
中科院分区：
文献类型：
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作者：
Kim, Deok-Ho;Lee, Hyojin;Levchenko, Andre
越来越明显的是，细胞生物学研究可以通过使用由纳米技术实现的生物工程工具而大大推进。纳米图案化技术的最新进展为控制从纳米到微米尺度的细胞相互作用的工程生物材料表面铺平了道路，允许更精确的定量实验捕获体外复杂组织生理学的多尺度方面。在纳米图案化表面上的细胞外信号线索的空间和时间控制显示（例如，例如，在一个实施例中，化学配体形式的线索、受控的硬度、纹理等）现在可以在生物学相关的长度尺度上实现，这是特别有吸引力的，使得能够实验平台，用于研究粘附介导的细胞信号传导的基本机制。在这里，我们提出了一个生物纳米图案化方法的概述，特别关注使用纳米纤维技术的最新进展，使工具研究细胞粘附和信号对细胞功能的影响。我们还强调了纳米工程在控制细胞-材料界面方面的影响，这对组织工程和再生医学的未来发展具有深远的意义。
It is becoming increasingly evident that cell biology research can be considerably advanced through the use of bioengineered tools enabled by nanoscale technologies. Recent advances in nanopatterning techniques pave the way for engineering biomaterial surfaces that control cellular interactions from the nano-to the microscale, allowing more precise quantitative experimentation capturing multi-scale aspects of complex tissue physiology in vitro. The spatially and temporally controlled display of extracellular signaling cues on nanopatterned surfaces (e. g., cues in the form of chemical ligands, controlled stiffness, texture, etc.) that can now be achieved on biologically relevant length scales is particularly attractive enabling experimental platform for investigating fundamental mechanisms of adhesion-mediated cell signaling. Here, we present an overview of bio-nanopatterning methods, with the particular focus on the recent advances on the use of nanofabrication techniques as enabling tools for studying the effects of cell adhesion and signaling on cell function. We also highlight the impact of nanoscale engineering in controlling cell-material interfaces, which can have profound implications for future development of tissue engineering and regenerative medicine.