Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas
Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas
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DOI：
10.1097/00001756-200403220-00001
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发表时间：
2004-03-22
期刊：
影响因子：
1.7
通讯作者：
Albrecht, J
中科院分区：
文献类型：
--
作者：
Sidoryk, M;Matyja, E;Albrecht, J
谷氨酰胺（Gln）是肿瘤组织中的生长决定因子。我们通过RT-PCR分析了编码Gln转运蛋白人类变体的mRNA的表达：ASCT 2（ASC系统）、SNAT 1 [ATA 1]（A系统）、SNAT 3 [SN 1]和SNAT 5 [SN 2]（N系统），这些基因存在于人类恶性胶质瘤WHO III/IV级（间变性星形细胞瘤和胶质母细胞瘤）、胶质瘤衍生的细胞培养物、外周器官脑转移瘤和对照脑组织样本中。（根据McKenzie和Ericksson最近提出的命名法指定的转运蛋白[1]。方括号内的原名称。SNAT 3 mRNA在胶质瘤中的表达比转移瘤或对照组织强3-5倍，并且在胶质瘤培养物中几乎不存在。天然胶质母细胞瘤用抗SNAT 3抗体免疫染色呈阳性。在所有研究的肿瘤组织中，ASCT 2 mRNA的表达增加，但SNAT 5或SNAT 1 mRNA的表达没有增加。因此，SNAT 3的表达增加是原位原发性恶性胶质瘤的标志物。
Glutamine (Gln) is a growth determinant in neoplastic tissues. We analysed by RT-PCR the expression of mRNAs coding for the human variants of Gln transporters: ASCT2 (system ASC), SNAT1 [ATA1] (system A), SNAT3 [SN1] and SNAT5 [SN2] (system N), in samples of human malignant gliomas WHO grades III/IV (anaplastic astrocytoma and glioblastoma), glioma-derived cell cultures, brain metastases from peripheral organs, and control brain tissue. (Transporters designated according to the nomenclature recently proposed by Mckenzie and Ericksson [1]. Original designations in square brackets.) SNAT3 mRNA showed a 3-5 times stronger expression in gliomas than in metastases or control tissue, and was virtually absent from glioma cultures. Native glioblastoma immunostained positively with anti-SNAT3 antibody. The expression of ASCT2 mRNA, but not SNAT5 or SNAT1 mRNAs, was increased in all neoplastic tissues studied. Hence, increased expression of SNAT3 is a marker of primary malignant gliomas in situ.