Quantitative analysis of axon collaterals of single cells in layer III of the piriform cortex of the guinea pig
Quantitative analysis of axon collaterals of single cells in layer III of the piriform cortex of the guinea pig
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DOI：
10.1002/cne.10844
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发表时间：
2003-10-20
影响因子：
2.5
通讯作者：
Kishi, K
中科院分区：
文献类型：
--
作者：
Chen, S;Murakami, K;Kishi, K
近期的生理学和形态学研究表明，梨状皮质（PC）的功能类似于新皮质的联合区，而非先前假设的典型初级感觉区。单细胞的轴突连接模式对于理解PC的功能组织非常重要。通过在豚鼠体内进行生物胞素细胞内注射，对PC的Ⅲ层中的3个单个锥体细胞和1个多棘多极细胞的轴突侧支进行了标记和定量分析。单个锥体细胞和多棘多极细胞在PC以及多个高阶行为/奖赏/情境相关区域（如前额叶皮质、杏仁核和内嗅皮质）内具有高度分布的轴突侧支，几乎没有斑块状聚集的趋势。对于锥体细胞，轴突侧支的平均长度为143毫米；平均突触小体数量为12930个。对于多棘多极细胞，轴突侧支的长度为88毫米；突触小体数量为7052个。PC前部（APC）的锥体细胞具有向前和向后方向的固有联合纤维，而PC后部（PPC）的锥体细胞和多棘多极细胞在PC内主要具有向后方向的固有联合纤维。我们的研究结果显示，Ⅲ层单细胞的连接模式类似于Ⅱ层锥体细胞的连接模式，这表明PC执行的相关功能类似于其他感觉系统联合区的功能。APC中从前到后的连接为循环过程提供了基础，而PPC中主要向后的连接则表明前馈过程占主导地位。（C）2003威利 - 利斯公司
Recent physiological and morphological studies suggest that the piriform cortex (PC) functions like the association areas of the neocortex rather than the typical primary sensory area as was previously assumed. The axon connection patterns of single cells are important for understanding the functional organization of the PC. The axon collaterals of three single pyramidal cells and one spiny multipolar cell in layer III of the PC were labeled and quantitatively analyzed by intracellular injections of biocytin in guinea pigs. The individual pyramidal and spiny multipolar cells have highly distributed axon collaterals, which display little tendency for patchy concentrations, within the PC and multiple higher order behavior/reward/contextual-related areas, such as the prefrontal cortex, amygdaloid nuclei, and entorhinal cortex. For the pyramidal cells, the average length of axonal collaterals is 143 mm; the average number of boutons is 12,930. For the spiny multipolar cell, the length of the axonal collaterals is 88 mm; the number of boutons is 7,052. The pyramidal cells in the anterior subdivision of the PC (APC) have both rostrally and caudally directed intrinsic association fibers, whereas the pyramidal and spiny multipolar cells in the posterior subdivision (PPC) have predominantly caudally directed intrinsic association fibers in the PC. Our results reveal that the connection patterns of single cells in layer III resemble those of pyramidal cells in layer II, suggesting that the PC performs correlative functions analogous to those in the association area of other sensory systems. The rostrally-to-caudally directed connections in the APC provide a substrate for the recurrent process, whereas largely caudally directed connections in the PPC suggest the dominance of the feed-forward process. (C) 2003 Wiley-Liss, Inc.