Nucleotides that contribute to the identity of Escherichia coli tRNA(Phe).
Nucleotides that contribute to the identity of Escherichia coli tRNA(Phe).
复制标题
有助于识别大肠杆菌 tRNA (Phe) 的核苷酸。
DOI：
10.1016/0022-2836(88)90551-7
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发表时间：
1988
影响因子：
5.6
通讯作者：
Foss,K
中科院分区：
文献类型：
--
作者：
McClain,WH;Foss,K
体外合成了tRNAPe琥珀抑制基因的一系列序列变体，并在大肠杆菌中克隆，以检测单个核苷酸对氨基酸接受性的贡献。构建了三种不同但互补类型的tRNA变体。第一个涉及E. colitRNAPhe。第二种类型的变异涉及基于枯草芽孢杆菌和火山盐杆菌中发现的野生型tRNAPhes序列的全基因合成。在第三类变体中，E. colitRNALys-tRNAPhe-tRNAPhe同源性的重要核苷酸序列在E.大肠杆菌位于L形tRNA分子的角落，在那里二氢尿苷环与T环相互作用，并延伸到反密码子茎的内部开口和相邻的可变环。连接角区和茎区的二氢尿苷茎区上的核苷酸序列尚未成功研究，尽管它可能有助于tRNAPheidentity。tRNAPhe 3′端第4个核苷酸对同一性有一定的意义。
A series of sequence variants of amber suppressor genes of tRNAPhewere synthesizedin vitroand cloned inEscherichia colito examine the contributions of individual nucleotides to identity for amino acid acceptance. Three different but complementary types of tRNA variants were constructed. The first involved the substitution of base-pairs on the cloverleaf stem regions of theE. colitRNAPhe. The second type of variant involved total gene synthesis based on wild-type tRNAPhesequences found inBacillus subtilisand inHalobacterium volcanii. In the third type of variant, the identity ofE. colitRNALyswas changed to that of tRNAPhe.The nucleotides which are important for tRNAPheidentity inE. coliare located on the corner of the L-shaped tRNA molecule, where the dihydrouridine loop interacts with the T loop, and extend to the interior opening of the anticodon stem and the adjoining variable loop. The nucleotide sequence on the dihydrouridine stem region, which joins the corner and stem regions, was not successfully studied though it may contribute to tRNAPheidentity. The fourth nucleotide from the 3′ end of tRNAPhehas some importance for identity.