Orexin 1 and orexin 2 receptor antagonism in the basolateral amygdala modulate long-term potentiation of the population spike in the perforant path-dentate gyrus-evoked field potential in rats
Orexin 1 and orexin 2 receptor antagonism in the basolateral amygdala modulate long-term potentiation of the population spike in the perforant path-dentate gyrus-evoked field potential in rats
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DOI：
10.1016/j.nlm.2018.02.024
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发表时间：
2018-03-01
影响因子：
2.7
通讯作者：
Akbari, Esmaeil
中科院分区：
文献类型：
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作者：
Ardeshiri, Motahareh Rouhi;Hosseinmardi, Narges;Akbari, Esmaeil
由于食欲素能系统缺陷导致的发作性睡病患者杏仁核-海马亚结构的受累，以及这种疾病中存在海马依赖的记忆障碍，我们研究了基底外侧杏仁核(BLA)的食欲素1和2受体拮抗剂对齿状回(DG)颗粒细胞长时程增强(LTP)的影响。我们在麻醉大鼠中使用了200赫兹的高频刺激方案。我们研究了强直刺激前后增食欲素受体失活后穿通径-齿状回颗粒细胞的长期突触可塑性。在BLA中存在增食欲素1和2受体拮抗剂(分别用SB-334867-A和TCS-OX2-29处理)时，DG群体峰电位的LTP与二甲基亚砜处理后的结果相比较，被减弱。然而，群体兴奋性突触后电位并未受到影响。此外，当LTP诱导后阻断BLA中的食欲素1和2受体时，DMSO组和治疗组之间没有差异。我们的研究结果表明，BLA的食欲素能系统在大鼠海马可塑性的调节中起着调节作用。
Involvement of amygdalo-hippocampal substructures in patients with narcolepsy due to deficiencies in the orexinergic system, and the presence of hippocampus-dependent memory impairments in this disorder, have led us to investigate the effects of orexin 1 and 2 receptor antagonism in the basolateral amygdala (BLA) on long-term potentiation (LTP) of dentate gyrus (DG) granular cells. We used a 200-Hz high-frequency stimulation protocol in anesthetized rats. We studied the long-term synaptic plasticity of perforant path-dentate gyrus granule cells following the inactivation of orexin receptors before and after tetanic stimulation. LTP of the DG population spike was attenuated in the presence of orexin 1 and 2 receptor antagonism (treatment with SB-334867-A and TCS-OX2-29, respectively) in the BLA when compared to that observed following treatment with dimethyl sulfoxide (DMSO). However, the population excitatory post-synaptic potentials were not affected. Moreover, when orexin 1 and 2 receptors in the BLA were blocked after LTP induction, there were no differences between the DMSO and treatment groups. Our findings suggest that the orexinergic system of the BLA plays a modulatory role in the regulation of hippocampal plasticity in rats.