Expression of Etk/Bmx tyrosine kinase in the tumorigenicity of nasopharyngeal. epithelium and its relation with EB virus infection and the apoptosis-related protein Bcl-2
Expression of Etk/Bmx tyrosine kinase in the tumorigenicity of nasopharyngeal. epithelium and its relation with EB virus infection and the apoptosis-related protein Bcl-2
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DOI：
10.1016/j.canlet.2005.02.028
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发表时间：
2006-02-08
期刊：
影响因子：
9.7
通讯作者：
Xiao, S
中科院分区：
文献类型：
--
作者：
Guo, LL;Guo, Y;Xiao, S
我们比较了鼻咽癌（NPC）和非肿瘤性鼻咽病变中Etk/Bmx的表达，以了解这种非受体蛋白酪氨酸激酶的表达是否与NPC的发生有关。我们还将 Etk/Bmx 表达与 Epstein-Barr 病毒 (EBV) 感染引起的因素相关联。我们使用免疫组织化学 (IHC) 和原位杂交检查了 20 个非肿瘤性鼻咽病变和 49 例鼻咽癌病例，以评估这些样本中 Etk/Bmx、EB 病毒潜伏膜蛋白-1 (LMP-1)、Bcl-2 和 EBV 编码的小 RNA-1 的表达。 1/9（11.1%）慢性鼻咽炎病例和2/11（18.2%）不典型增生病例的鼻咽上皮基底细胞核中存在Etk/Bmx表达。虽然13/49（26.5%）鼻咽癌病例表达Etk/Bmx，但非肿瘤病例和鼻咽癌病例中Etk/Bmx的表达频率差异不显着。 Etk/Bmx 表达与不典型增生和鼻咽癌中 EBER-1 免疫阳性的存在相关，但与慢性鼻咽炎无关。 Etk/Bmx 免疫阳性的存在与鼻咽癌或非肿瘤性病变中 LMP-1 或 Bcl-2 的表达无关。这提示在某些非肿瘤性和肿瘤性鼻咽病变病例中，Etk/Bmx可能参与调节上皮分化。虽然 EBV 相关的小 RNA-I 可能参与这种调节，但 LMP-1 或 Bcl-2 表达似乎与 Etk/Bmx 表达无关。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
We compared Etk/Bmx expression in nasopharyngeal carcinoma (NPC) and non-neoplastic nasopharyngeal lesions in order to learn whether the expression of this non-receptor protein tyrosine kinase is associated with the development of NPC. We also related Etk/Bmx expression to factors resulting from Epstein-Barr virus (EBV) infection. We used immunohistochemistry (IHC) and in situ hybridization to examine 20 non-neoplastic nasopharyngeal lesions and 49 cases of NPC to assess Etk/Bmx, EB virus latent membrane protein-1 (LMP-1), Bcl-2 and EBV-encoded small RNA-1 expression in these samples. Etk/Bmx expression was present in the basal cell nuclei of the nasopharyngeal epithelium in 1/9 (11.1%) cases of chronic nasopharyngitis and 2/11 cases (18.2%) of dysplasia. While 13/49 (26.5%) NPC cases expressed Etk/Bmx, the difference in frequency between the expression of Etk/Bmx in the non-neoplastic and NPC cases was not significant. Etk/Bmx expression was correlated with the presence of EBER-1 immunopositivity in dysplasia and in NPC but not in chronic nasopharyngitis. The presence of Etk/Bmx immunopositivity was independent of the expression of either LMP-1 or Bcl-2 in either the nasopharyngeal carcinoma or the non-neoplastic lesions. This suggests that in some cases of non-neoplastic and neoplastic nasopharyngeal lesions, Etk/Bmx may participate in regulating epithelial differentiation. While EBV-related small RNA-I may participate in this regulation, neither LMP-1 or Bcl-2 expression appears to be related to Etk/Bmx expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.