A screen for targets of the Xenopus T-box gene Xbra
A screen for targets of the Xenopus T-box gene Xbra
复制标题
非洲爪蟾T-box基因Xbra靶标的筛选
DOI：
10.1016/s0925-4773(00)00260-4
复制
发表时间：
2000
影响因子：
2.6
通讯作者：
J. Smith
中科院分区：
文献类型：
--
作者：
Y. Saka;M. Tada;J. Smith
Brachyury (T), a member of the T-box gene family, is essential for the formation of posterior mesoderm and notochord in vertebrate development. Expression of the Xenopus homologue of Brachyury, Xbra, causes ectopic ventral and lateral mesoderm formation in animal cap explants and co-expression of Xbra with Pintallavis, a forkhead/HNF3β-related transcription factor, induces notochord. Although eFGF and the Bix genes are thought to be direct targets of Xbra, no other target genes have been identified. Here, we describe the use of hormone-inducible versions of Xbra and Pintallavis to construct cDNA libraries enriched for targets of these transcription factors. Five putative targets were isolated: Xwnt11, the homeobox gene Bix1, the zinc-finger transcription factor Xegr-1, a putative homologue of the antiproliferative gene BTG1 called Xbtg1, and BIG3/1A11, a gene of unknown function. Expression of Xegr-1 and Xbtg1 is controlled by Pintallavis alone as well as by a combination of Xbra and Pintallavis. Overexpression of Xbtg1 perturbed gastrulation and caused defects in posterior tissues and in notochord and muscle formation, a phenotype reminiscent of that observed with a dominant-negative version of Pintallavis called Pintallavis-EnR. The Brachyury-inducible genes we have isolated shed light on the mechanism of Brachyury function during mesoderm formation. Specification of mesodermal cells is regulated by targets including Bix1–4 and eFGF, while gastrulation movements and perhaps cell division are regulated by Xwnt11 and Xbtg1.
影响因子：
4.6
作者：
C. L. Olsen;William R. Jeffery
通讯作者：
C. L. Olsen;William R. Jeffery
影响因子：
1.6
作者：
Hendzel, MJ;Wei, Y;Allis, CD
通讯作者：
Allis, CD
Bix4 直接被 VegT 激活并介导非洲爪蟾发育中内胚层的形成。
DOI：
10.1242/dev.126.19.4193
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Casey,ES;Tada,M;Fairclough,L;Wylie,CC;Heasman,J;Smith,JC
通讯作者：
Smith,JC