Synthesis and characterization of novel polar-embedded silica stationary phases for use in reversed-phase high-performance liquid chromatography
Synthesis and characterization of novel polar-embedded silica stationary phases for use in reversed-phase high-performance liquid chromatography
复制标题
用于反相高效液相色谱的新型极性嵌入二氧化硅固定相的合成和表征
DOI：
10.1016/j.chroma.2012.11.045
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发表时间：
2013-01-04
影响因子：
4.1
通讯作者：
Wen, Aidong
中科院分区：
文献类型：
--
作者：
Wang, Haibo;Chen, Lei;Wen, Aidong
通过一种简单有效的合成方法，研制了一系列新型C-10二肽固定相。新相的制备包括硅烷的合成和二氧化硅的表面改性。使用Engelhardt、Tanaka和Neue供试混合物对这些色谱柱进行色谱评价。这些新的固定相的适用性也进行了评估，使用一系列的诊断探针，包括酸，碱或中性化合物和几个通用的应用程序。这些新的C-10二肽固定相在较宽的pH范围内显示出优异的水解稳定性。与其他现有的酰胺嵌入柱一样，这些新的固定相与传统的C-18柱相比，对极性和亲水性化合物具有更高的保留率和不同的选择性。这些新相与100%水移动的相相容，并且还为酸性和碱性化合物提供高柱效和良好的峰形。(C)2012爱思唯尔有限公司版权所有。
We have developed a series of new C-10 dipeptide stationary phases via a simple and effective synthetic method. The preparation of the new phases involves the synthesis of silanes and the surface modification of silica. Chromatographic evaluations of these columns were performed using the Engelhardt, Tanaka, and Neue test mixtures. The applicability of these new stationary phases was also evaluated using a series of diagnostic probes including acids, bases or neutral compounds and several generic applications. These new C-10 dipeptide stationary phases showed excellent hydrolytic stability over a wide pH range. Like other existing amide-embedded columns, these new stationary phases exhibit higher retention for polar and hydrophilic compounds and different selectivity as compared to conventional C-18 columns. These new phases are compatible with 100% aqueous mobile phases, and also provide high column efficiency and good peak shapes for both acidic and basic compounds. (C) 2012 Elsevier B.V. All rights reserved.