A PERSISTENT UNTRANSLATED SEQUENCE WITHIN BACTERIOPHAGE-T4 DNA TOPOISOMERASE GENE-60
A PERSISTENT UNTRANSLATED SEQUENCE WITHIN BACTERIOPHAGE-T4 DNA TOPOISOMERASE GENE-60
复制标题
DOI：
10.1126/science.2830666
复制
发表时间：
1988-02-26
期刊：
影响因子：
56.9
通讯作者：
FANG, M
中科院分区：
文献类型：
--
作者：
HUANG, WM;AO, SZ;FANG, M
大肠杆菌噬菌体 T4 基因 60 的编码序列中存在 50 个核苷酸的非翻译区，该基因编码其 II 型 DNA 拓扑异构酶的亚基之一。这种中断是转录信使 RNA 的一部分，并且在翻译前似乎不会被去除。因此，信使 RNA 和编码的蛋白质序列之间通常存在的共线性在这种情况下显然不存在。中断由五个碱基对的直接重复括起来。提出了一种机制，其中非翻译区的折叠将由中断分开的密码子聚集在一起，以便延伸的核糖体可以在翻译过程中跳过 50 个核苷酸。另一种可能性是，该蛋白质是从一种非常微小且不可检测的加工过的信使 RNA 中有效翻译而来的，这种可能性似乎不太可能，但尚未完全排除。
A 50-nucleotide untranslated region is shown to be present within the coding sequence of Escherichia coli bacteriophage T4 gene 60, which encodes one of the subunits for its type II DNA topoisomerase. This interruption is part of the transcribed messenger RNA and appears not to be removed before translation. Thus, the usual colinearity between messenger RNA and the encoded protein sequence apparently does not exist in this case. The interruption is bracketed by a direct repeat of five base pairs. A mechanism is proposed in which folding of the untranslated region brings together codons separated by the interruption so that the elongating ribosome may skip the 50 nucleotides during translation. The alternative possibility, that the protein is efficiently translated from a very minor and undectable form of processed messenger RNA, seems unlikely, but has not been completely ruled out.