Accurate and independent measurement of volume and hemoglobin concentration of individual red cells by laser light scattering.
Accurate and independent measurement of volume and hemoglobin concentration of individual red cells by laser light scattering.
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通过激光散射准确、独立地测量单个红细胞的体积和血红蛋白浓度。
作者：
N. Mohandas;Y. R. Kim;D. Tycko;J. Orlik;J. Wyatt;W. Groner
红细胞体积(MCV)和血红蛋白浓度(MCHC)是用来表征贫血患者血液的红细胞指数。自从引入流式细胞术方法来测量这些指标以来，人们普遍认为这些仪器得出的值是准确的。然而，最近的研究表明，许多细胞因素，包括细胞变形性的改变，可能会导致这些自动化仪器对细胞体积的不准确测量。由于细胞血红蛋白浓度和红细胞压积是根据细胞体积的测量值计算的，因此这些指标的准确性也会因为细胞体积的不准确而受到影响。本研究采用一种基于激光光散射的流式细胞术实验方法，测量了密度分级的正常和镰状红细胞、脱水和脱水的正常红细胞以及各种病理细胞的MCV和MCHC，该方法可以独立测量体积和血红蛋白浓度。我们发现，新方法在MCV(30到120 fl)和MCHC(27到45 g/dL)的大范围内准确地测量体积和血红蛋白浓度。这与目前可用的方法不同，在这些方法中，可以在更有限的范围内(27至35g/dL)准确测量血红蛋白浓度。此外，由于实验方法独立测量单个红细胞的体积和血红蛋白浓度，因此可以生成体积和血红蛋白浓度分布的直方图，并推导出体积分布的变异系数和血红蛋白浓度分布的标准差。我们已经能够证明，在病理性红细胞样本中，体积和血红蛋白浓度分布可以相互独立地变化。
Cell volume (MCV) and hemoglobin concentration (MCHC) are the red cell indices used to characterize the blood of patients with anemia. Since the introduction of flow cytometric methods for the measurement of these indices, it has generally been assumed that the values derived by these instruments are accurate. However, it has recently been shown that a number of cellular factors, including alterations in cellular deformability, can lead to inaccurate measurement of cell volume by these automated instruments. Because cell hemoglobin concentration and hematocrit are computed from the measured values of cell volume, accuracy of these indices is also compromised by inaccurate determination of cell volume. A recently developed experimental flow cytometric method based on laser light scattering, which can independently measure volume and hemoglobin concentration, has been used in the present study to measure MCV and MCHC of density-fractionated normal and sickle red cells, hydrated and dehydrated normal red cells, and various pathologic cells. We found that the new method accurately measures both volume and hemoglobin concentrations over a wide range of MCV (30 to 120 fL) and MCHC (27 to 45 g/dL) values. This is in contrast to currently available methods in which hemoglobin concentration values are accurately measured over a more limited range (27 to 35 g/dL). In addition, as the experimental method independently measures volume and hemoglobin concentration of individual red cells, it allowed us to generate histograms of volume and hemoglobin concentration distribution and derive coefficient of variation for volume distribution and standard deviation of hemoglobin concentration distribution. We have been able to document that volume and hemoglobin concentration distributions can vary independently of each other in pathologic red cell samples.
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镰状细胞性贫血痛苦危机的一个客观迹象是：高密度红细胞随之减少。
影响因子：
20.3
作者：
Fabry,ME;Benjamin,L;Lawrence,C;Nagel,RL
通讯作者：
Nagel,RL
镰状细胞中红细胞的异质性：具有实际临床和病理生理学意义的特征。
DOI：
--
发表时间：
1982
期刊：
Blood cells
影响因子：
--
作者：
Fabry,ME;Nagel,RL
通讯作者：
Nagel,RL
轻型地中海贫血：常规红细胞测量和与缺铁的鉴别。
影响因子：
3.5
作者：
Johnson,CS;Tegos,C;Beutler,E
通讯作者：
Beutler,E
影响因子：
15.9
作者：
EVANS, E;MOHANDAS, N;LEUNG, A
通讯作者：
LEUNG, A