CORTISOL DIRECTLY STIMULATES DIFFERENTIATION OF CHLORIDE CELLS IN TILAPIA OPERCULAR MEMBRANE
CORTISOL DIRECTLY STIMULATES DIFFERENTIATION OF CHLORIDE CELLS IN TILAPIA OPERCULAR MEMBRANE
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DOI：
10.1152/ajpregu.1990.259.4.r857
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发表时间：
1990-10-01
影响因子：
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通讯作者：
MCCORMICK, SD
中科院分区：
文献类型：
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作者：
MCCORMICK, SD
淡水罗非鱼（Oreochromis mossambicus）的眼膜在体外维持4天，并暴露于不同浓度的皮质醇（0、0.01、0.1、1和10 μ g/ml）中。氯细胞的大小、数量和Na+-K+- atp酶含量采用荧光线粒体染料（二甲氨基苯基乙基吡啶碘）、一种与Na+-K+- atp酶特异性结合的瓦巴因（anthroylouabain）的荧光类似物和一种对血浆和管状膜特异性的细胞学染色进行检测。在缺乏皮质醇的情况下，淡水罗非鱼眼膜氯细胞密度下降（从初始水平6114 +-）。451到18 .+-。9个细胞/cm2)，并以剂量依赖的方式被皮质醇恢复。氯离子槽高度95.5 +-。0.3 .mu。初始值为M， +- 7.8。0.5 .mu。体外4 d后，M增加了2倍(13.1 +-。0.7 .mu。M)暴露于1 .mu后。g / ml皮质醇。在对照培养基中初始和4天后，没有检测到虫鲁巴因的染色；暴露在1亩。G /ml皮质醇导致大量anthroylouabain阳性氯细胞出现。在不添加皮质醇的情况下，眼膜Na+-K+- atp酶活性在培养4天后保持不变（0.4-0.6 .mu）。mol ADP.cntdot.mg protein-1.cntdot.h-1)；皮质醇的加入引起了剂量依赖性的增加，最大可达1.2。0.1 .mu。蛋白质1.cntdot.h-1。在体外，皮质醇还能维持海水适应罗非鱼眼膜氯细胞的大小、密度和外观。结果表明，体外皮质醇暴露导致氯细胞海水形态的形态和生化分化。
Opercular membranes from freshwater tilapia (Oreochromis mossambicus) were maintained in vitro for 4 days and exposed to several concentrations of cortisol (0, 0.01, 0.1, 1, and 10 .mu.g/ml). Chloride cell size, number, and Na+-K+-ATPase content were examined using a fluorescent mitochondrial dye (dimethylaminostyrylethylpyridiniumiodine), a fluorescent analogue of ouabain (anthroylouabain) that binds specifically to Na+-K+-ATPase, and a cytological stain specific for plasma and tubular membranes. In the absence of cortisol, chloride cell density of the freshwater tilapia opercular membrane decreased (from initial levels of 6,114 .+-. 451 to 18 .+-. 9 cells/cm2) and was restored by cortisol in a dose-dependent manner. Chloride cell height 95.5 .+-. 0.3 .mu.m initially and 7.8 .+-. 0.5 .mu.m after 4 days in vitro) increased twofold (13.1 .+-. 0.7 .mu.m) after exposure to 1 .mu.g/ml cortisol. Initially and after 4 days in control medium, there was no detectable staining with anthroylouabain; exposure to 1 .mu.g/ml cortisol resulted in the appearance of numerous anthroylouabain-positive chloride cells. Without cortisol, Na+-K+-ATPase activity of the opercular membrane remained constant through 4 days of culture (0.4-0.6 .mu.mol ADP.cntdot.mg protein-1.cntdot.h-1); addition of cortisol caused a dose-dependent increase to a maximum of 1.2 .+-. 0.1 .mu.mol Pi.cntdot.mg protein-1.cntdot.h-1. In vitro cortisol also maintained the size, density, and appearance of chloride cells from opercular membrane of seawater-adpated tilapia. The results indicate that in vitro cortisol exposure causes morphological and biochemical differentiation of the seawater form of the chloride cell.