TRANSCRIPTION OF DNA INJECTED INTO XENOPUS OOCYTES IS INFLUENCED BY TEMPLATE TOPOLOGY
TRANSCRIPTION OF DNA INJECTED INTO XENOPUS OOCYTES IS INFLUENCED BY TEMPLATE TOPOLOGY
复制标题
DOI：
10.1038/302038a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
--
作者：
HARLAND, RM;WEINTRAUB, H;MCKNIGHT, SL
将单纯疱疹病毒胸苷激酶注入非洲爪蟾卵母细胞后，在环形和线性质粒上测定其转录。环状质粒似乎比线性质粒转录效率高至少500倍。当允许环状模板形成功能性转录复合物，然后在原位线性化时，准确转录物的合成大大减少。
The transcription of the herpes simplex virus thymidine kinase was assayed on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. When the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.