THE EFFECT OF PGE1 AND TEMPERATURE ON LUNG FUNCTION FOLLOWING PRESERVATION
THE EFFECT OF PGE1 AND TEMPERATURE ON LUNG FUNCTION FOLLOWING PRESERVATION
复制标题
PGE1 和温度对保存后肺功能的影响
作者：
T. Ueno;H. Yokomise;T. Oka;J. Puskas;E. Mayer;Arthur S Slutsky;G. Patterson
我们在离体兔肺灌注模型中研究了血管扩张剂（前列腺素E1）以及冲洗(F)和储存(S)温度（4°C或10°C）对肺保存的影响。冲洗液采用低钾葡聚糖（LPD）或Euro-Collins （E-C）溶液。研究共6组，共6只动物：1组（LPD, 4°C F-S）、2组（LPD合并PGE， 4°C F-S）、3组（E-C合并PGE， 4°C F-S）、4组（LPD, 10°C F-S）、5组（LPD合并PGE1, 10°C F-S）、6组（E-C合并PGE1, 10°C F-S）。保存18小时后，单独左肺通气，再灌注新鲜静脉血。测定再灌注时PaO2、PaCO2、肺动脉压（PAP）、气管压（P1）、湿重/干重（W/D）比。在4°C（1组95.8±11.5 mmHg， 2组102.7±8.6 mmHg， 3组41.8±10.5 mmHg， P>0.01）和10°C（4组119.3±2.3 mmHg， 5组131.1±6.2 mmHg， 6组54.6±5.2，P>0.01）时，LPD伴PGE1或不伴PGE1的PaO2均显著高于伴PGEi的E-C。LPD组PaCO2、PAP、P1、W/D比值均低于E-C组。LPD/PGE1和单独LPD的肺保存效果相似。经LPD冲洗后保存在10°C的肺PaO2高于保存在4°C的肺。我们得出结论，即使使用PGE1， LPD溶液在离体兔肺保存模型中也优于E-C溶液。中等剂量的PGE1不能改善LPD作为冲洗液的性能。LPD在10°C下的肺保存优于在4°C下的保存。
We studied the effect of a vasodilator (prostaglandin E1) as well as flush (F) and storage (S) temperatures (4°C or 10°C) on lung preservation in an isolated rabbit lung perfusion model. Low-potassium dextran (LPD) or Euro-Collins (E-C) solution was used as flush solution. Six groups of six animals were studied: group 1 (LPD, 4°C F-S), group 2 (LPD with PGE, 4°C F-S), group 3 (E-C with PGE, 4°C F-S), group 4 (LPD, 10°C F-S), group 5 (LPD with PGE1, 10°C F-S), group 6 (E-C with PGE1, 10°C F-S). After 18-hr preservation, left lungs alone were ventilated, and reperfused with fresh venous blood. PaO2, PaCO2, pulmonary artery pressure (PAP), tracheal pressure (P1) during reperfusion, and wet/dry weight (W/D) ratios were measured. PaO2 after LPD with or without PGE1 was significantly higher than after E-C with PGEi at 4°C (95.8±11.5 mmHg in group 1 or 102.7±8.6 in group 2 vs. 41.8±10.5 in group 3, P>0.01) and at 10°C (119.3±2.3 in group 4 or 131.1± 6.2 in group 5 vs. 54.6±5.2 in group 6, P>0.01). PaCO2, PAP, P1, and W/D ratios in the LPD groups were lower than in the E-C groups. LPD/PGE1 and LPD alone produced similar pulmonary preservation. PaO2 of lungs flushed with LPD and preserved at 10°C was higher than that of lungs stored at 4°C. We conclude that LPD solution is superior to E-C solution in this ex vivo rabbit lung preservation model, even when PGE1 is used. A moderate dose of PGE1 did not improve the performance of LPD as a flush solution. Pulmonary preservation with LPD at 10°C is superior to preservation at 4°C.