Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice
Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice
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DOI：
10.1073/pnas.96.13.7575
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发表时间：
1999-06-22
影响因子：
11.1
通讯作者：
Iwasaki, Y
中科院分区：
文献类型：
--
作者：
Fujisawa, Y;Kato, T;Iwasaki, Y
含有水稻异源三聚体G蛋白α -亚基反义cDNA的转基因水稻很少或不产生该亚基的mRNA，并且表现出异常的形态，包括矮化性状和小种子。在正常水稻中，a亚基的mRNA在节间和小花中丰富，这些组织与矮秆转化体的异常密切相关。利用限制性内切片段长度多态性定位了该基因在水稻第5染色体上的位置。该位置与水稻矮秆突变体Dailioku dwarf （d-1）的基因座密切相关，已知该突变体表现出与通过反义技术抑制a亚基内源mRNA的转化子相似的异常表型。对大田矮子（d-1）、ID-1、DK22、DKT-1、DKT-2和CM1361-1 5个等位基因α -亚基的cdna分析表明，这些矮子突变体在α -亚基基因编码区发生了突变。这些结果表明，G蛋白在水稻正常节间和种子的形成中起作用。
Transgenic rice containing an antisense cDNA for the alpha- subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the a subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the alpha-subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Dailioku dwarf (d-1), which is known,vn to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the a subunit by antisense technology. Analysis of the cDNAs for the alpha- subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the alpha-subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.