The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum have short A tails
The fragmented mitochondrial ribosomal RNAs of Plasmodium falciparum have short A tails
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DOI：
10.1093/nar/27.11.2416
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发表时间：
1999-06-01
影响因子：
14.9
通讯作者：
Feagin, JE
中科院分区：
文献类型：
--
作者：
Gillespie, DE;Salazar, NA;Feagin, JE
恶性疟原虫（Plasmodiumfalciparum）线粒体基因组编码高度片段化的rRNA，目前已发现20个小RNA片段，其中15个小RNA片段与rRNA序列的特定区域相对应。为了研究核糖体中片段化rRNA之间可能的相互作用，我们使用引物延伸和RNase保护分析绘制了许多小转录本的末端。从这些研究中获得的结果显示，一些rRNA转录本比编码它们的序列更长。为了研究这些大小差异，我们进行了3'RACE PCR分析和RNase H作图。这些分析揭示了在一些但不是所有这些小rRNA上的非编码寡核苷酸（A）尾。寡核苷酸（A）尾的近似长度似乎是转录物特异性的，其中一些rRNA一致地显示出比其他rRNA更长的寡核苷酸（A）尾。rRNA的寡腺苷酸化可以提供针对3'核酸外切攻击的缓冲区，从而保留核糖体中二级结构相互作用所必需的编码序列。
The mitochondrial genome of Plasmodium falciparum encodes highly fragmented rRNAs, Twenty small RNAs which are putative rRNA fragments have been found and 15 of them have been identified as corresponding to specific regions of rRNA sequence. To investigate the possible interactions between the fragmented rRNAs in the ribosome, we have mapped the ends of many of the small transcripts using primer extension and RNase protection analysis. Results obtained from these studies revealed that some of the rRNA transcripts were longer than the sequences which encode them. To investigate these size discrepancies, we performed 3' RACE PCR analysis and RNase H mapping. These analyses revealed non-encoded oligo(A) tails on some but not all of these small rRNAs, The approximate length of the oligo(A) tail appears to be transcript-specific, with some rRNAs consistently showing longer oligo(A) tails than others. The oligoadenylation of the rRNAs may provide a buffer zone against 3' exonucleolytic attack, thereby preserving the encoded sequences necessary for secondary structure interactions in the ribosome.