Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts
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DOI：
10.1016/j.jep.2012.10.029
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发表时间：
2012-12-18
影响因子：
5.4
通讯作者：
Boylan, Fabio
中科院分区：
文献类型：
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作者：
Abdul-Wahab, Ikarastika Rahayu;Guilhon, Carolina Carvalho;Boylan, Fabio
民族药理学相关性：马来西亚当地社区食用 Pereskia bleo Kunth。 （仙人掌科）叶子作为生蔬菜或作为混合物和茶饮用，可治疗糖尿病、高血压、风湿病、癌症相关疾病、炎症、胃痛、溃疡，并可恢复身体活力。研究目的：在两种镇痛模型中评估该物种首次分离的提取物和牡荆素的抗伤害活性；福尔马林诱导的舔舐和乙酸诱导的腹部扭动。材料和方法：使用索氏提取器用乙醇提取三公斤半的P.bleo叶，提取72小时。将粗乙醇提取物用活性炭处理过夜并进行液-液分配，得到己烷、二氯甲烷、乙酸乙酯和丁醇提取物。除了进行急性毒性测定外，还使用福尔马林试验和乙酸诱导的腹部扭动测试了所有提取物（包括从乙酸乙酯分区中分离出的粗乙醇和牡荆素）的外周抗伤害活性。结果：植物化学分析结果分别从乙酸乙酯、二氯甲烷和己烷提取物中分离出牡荆素 (1)、β-谷甾醇葡萄糖苷 (2) 和 β-谷甾醇 (3)。这是首次从该物种中分离出牡荆素和β-谷甾醇葡萄糖苷。所有提取物的抗伤害活性仅为中等。从乙酸乙酯提取物中分离出的牡荆素，在以与提取物中相同的浓度单独使用时，在所有测试的模型中均未表现出任何活性。结论：本研究表明，所有提取物都具有中等的抗伤害活性。牡荆素不是乙酸乙酯提取物中产生抗伤害作用的化合物。需要进一步研究以确定可能负责该植物抗伤害活性的化合物。 c 2012 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Local communities in Malaysia consume Pereskia bleo Kunth. (Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes, hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitalizing the body.Aim of the study: To evaluate anti-nociceptive activity of the extracts and vitexin, isolated for the first time in this species, in two analgesic models; formalin-induced licking and acetic acid-induced abdominal writhing.Materials and methods: Three and a half kilos of P. bleo leaves were extracted using Soxhlet apparatus with ethanol for 72 h. The crude ethanol extract was treated with activated charcoal overnight and subjected to a liquid-liquid partition yielding hexane, dichloromethane, ethyl acetate and butanol extracts. All extracts, including the crude ethanol and vitexin isolated from the ethyl acetate partition were tested for peripheral anti-nociceptive activity using formalin test and acetic acid-induced abdominal writhing, besides having their acute toxicity assays performed. Results: The phytochemical analyses resulted in the isolation of vitexin (1), beta-sitosterol glucoside (2) and beta-sitosterol (3) isolated from the ethyl acetate, dichloromethane and hexane extracts, respectively. This is the first time vitexin and beta-sitosterol glucoside are isolated from this species. The anti-nociceptive activities for all extracts were only moderate. Vitexin, which was isolated from the ethyl acetate extract did not show any activity in all models tested when used alone at the same concentration as it appears in the extract.Conclusion: This study showed that all the extracts possess moderate anti-nociceptive activity. Vitexin is not the compound responsible for the anti-nociceptive effect in the ethyl acetate extract. Further investigations are needed to identify the compound(s) that might be responsible for the anti-nociceptive activity in this plant. c 2012 Elsevier Ireland Ltd. All rights reserved.