Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization
Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization
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DOI：
10.1128/aem.66.12.5116-5122.2000
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Kirchman, DL
中科院分区：
文献类型：
--
作者：
Cottrell, MT;Kirchman, DL
我们利用16S rRNA基因克隆文库和荧光原位杂交技术(FISH)测定了加州沿海表层水中浮游细菌群落的组成，以比较这两种非培养方法得出的群落结构。两个克隆文库的组成与前人研究的海洋浮游细菌克隆文库的组成非常相似。来自伽马蛋白细菌的克隆约占文库的28%，而约55%的克隆来自占克隆文库主导地位的cr-蛋白细菌。细胞吞噬-黄杆菌组和其他三个组各占克隆文库的10%或更少。FISH确定的群落组成与克隆文库所暗示的组成有很大的不同。在FISH分析的II群落中，有8个群落以细胞吞噬-黄杆菌群为主，包括用克隆文库分析的两个群落。用α-蛋白细菌探针FISH平均只有10%的DAPI(4‘，6’-二氨基-2-苯基吲哚)染色的细菌被FISH检测到，但根据FISH的确定，30%的DAPI染色的细菌似乎属于细胞吞噬黄杆菌组。与FISH测定的相对丰度相比，克隆文库中OL-Protebacteria的相对丰度显著偏高，而Cytophaga-黄杆菌在克隆文库中的相对丰度较低。我们的数据表明，细胞吞噬-黄杆菌类群在数量上可能是沿海海洋浮游细菌群落的主要组成部分。
We determined the compositions of bacterioplankton communities in surface waters of coastal California using clone libraries of 16S rRNA genes and fluorescence in situ hybridization (FISH) in order to compare the community structures inferred from these two culture-independent approaches. The compositions of two clone libraries were quite similar to those of clone libraries of marine bacterioplankton examined by previous studies. Clones from gamma -proteobacteria comprised ca. 28% of the libraries, while approximately 55% of the clones came from cr-proteobacteria, which dominated the clone libraries. The Cytophaga-Flavobacter group and three others each comprised 10% or fewer of the clone libraries. The community composition determined by FISH differed substantially from the composition implied by the clone libraries. The Cytophaga-Flavobacter group dominated 8 of the II communities assayed by FISH, including the two communities assayed using clone libraries. On average only 10% of DAPI (4',6'-diamidino-2-phenylindole)-stained bacteria were detected by FISH with a probe for alpha -proteobacteria, but 30% of DAPI-stained bacteria appeared to be in the Cytophaga-Flavobacter group as determined by FISH. ol-Proteobacteria were greatly overrepresented in clone libraries compared to their relative abundance determined by FISH, while the Cytophaga-Flavobacter group was underrepresented in clone libraries. Our data show that the Cytophaga-Flavobacter group can be a numerically dominant component of coastal marine bacterioplankton communities.