Induction of synthesis of the cytolytic C9 (ninth component of complement)-related protein in human peripheral mononuclear cells by monoclonal antibody OKT3 or interleukin 2: correlation with cytotoxicity and lymphocyte phenotype.
Induction of synthesis of the cytolytic C9 (ninth component of complement)-related protein in human peripheral mononuclear cells by monoclonal antibody OKT3 or interleukin 2: correlation with cytotoxicity and lymphocyte phenotype.
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单克隆抗体 OKT3 或白细胞介素 2 诱导人外周单核细胞合成溶细胞 C9（补体第九种成分）相关蛋白：与细胞毒性和淋巴细胞表型的相关性。
DOI：
10.1073/pnas.84.9.2946
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发表时间：
1987
影响因子：
11.1
通讯作者：
Müller-Eberhard,HJ
中科院分区：
文献类型：
--
作者：
Martin,DE;Zalman,LS;Jung,G;Müller-Eberhard,HJ
细胞溶解c9相关蛋白（C9RP）的合成是通过抗cd3抗体OKT3或白细胞介素2激活静息的人外周血T淋巴细胞来诱导的。活化过程中不同时间细胞毒性与C9RP含量的相关系数r = 0.92。在OKT3刺激外周单核细胞时，C9RP含量和细胞毒性在第2天或第3天达到最大值，随后在第5天下降到基线值，而在白细胞介素2刺激时，这两个参数在第3-5天达到最大值。荧光活化细胞分选后，定量CD4+、CD8+和Leu-19+亚群的C9RP和细胞毒性。在okt3激活的CD8+细胞中，C9RP增加到每个细胞约3 X 10(6)分子，抗cd3 -抗黑色素瘤单克隆抗体偶联物介导的人黑色素瘤细胞裂解相应增加。白细胞介素2刺激的CD8+细胞表现出类似的增加，但细胞毒性与偶联物无关。活化的CD4+细胞显示C9RP含量的轻微增加。u-19+细胞表现出自然杀伤细胞活性，在刺激前具有较高的C9RP含量（每个细胞约2.5 X 10(6)分子）。
Synthesis of the cytolytic C9-related protein (C9RP) was induced by activation of resting human peripheral T lymphocytes with the anti-CD3 antibody OKT3 or interleukin 2. Comparison of cellular cytotoxicity and C9RP content at various times during activation yielded a coefficient of correlation r = 0.92. During OKT3 stimulation of peripheral mononuclear cells, maximal C9RP content and cytotoxicity were observed by day 2 or 3, with subsequent decline to baseline values by day 5, whereas during interleukin 2 stimulation, both parameters reached the maximal level at days 3-5. After fluorescence-activated cell sorting, C9RP and cytotoxicity were quantitated in CD4+, CD8+, and Leu-19+ subsets. In OKT3-activated CD8+ cells, C9RP increased to approximately 3 X 10(6) molecules per cell, with a corresponding increase in lysis of human melanoma cells mediated by anti-CD3-anti-melanoma monoclonal antibody conjugates. Interleukin 2-stimulated CD8+ cells showed similar increases, but cytotoxicity was conjugate-independent. Activated CD4+ cells showed minimal increase in C9RP content. Leu-19+ cells, which exhibit natural killer cell activity, had a high C9RP content (approximately 2.5 X 10(6) molecules per cell) before stimulation.