Autofluorescence and Diffuse Reflectance Spectroscopy and Spectral Imaging for Breast Surgical Margin Analysis
Autofluorescence and Diffuse Reflectance Spectroscopy and Spectral Imaging for Breast Surgical Margin Analysis
复制标题
DOI：
10.1002/lsm.20865
复制
发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Mahadevan-Jansen, Anita
中科院分区：
文献类型：
--
作者：
Keller, Matthew D.;Majumder, Shovan K.;Mahadevan-Jansen, Anita
背景和目的：大多数患有早期乳腺癌的女性可以选择乳房保留治疗，这涉及部分乳房切除术以切除原发肿瘤，通常随后进行放射治疗。切缘处或附近的肿瘤存在与局部肿瘤复发的风险密切相关，因此需要一种非侵入性的实时工具来评估切缘状态。本研究探讨了使用自体荧光和漫反射光谱和光谱成像，以评估利润率状态intraoperating.Materials和方法：光谱测量的组织块的表面后立即去除部分乳房切除术和/或组织切片后立即手术病理。结果：经与组织病理学相关分析，多元统计算法将光谱分为正常（阴性边缘）和肿瘤（阳性边缘），其敏感性为85%，特异性为96%。单独的算法实现了新辅助化疗治疗组织和未治疗组织之间的100%分类。荧光和反射率为基础的光谱图像能够划定一个钙化病变的表面上切除的标本，以及.Conclusion：荧光和反射光谱可以是一个有价值的工具，用于检查切除的乳腺肿瘤标本的浅表边缘状态，特别是在光谱成像的形式，检查整个利润率在一个单一的收购。激光外科医学42：1523，2010。(C)2010 Wiley-Liss，Inc.
Background and Objective: Most women with early stage breast cancer have the option of breast conserving therapy, which involves a partial mastectomy for removal of the primary tumor, usually followed by radiotherapy. The presence of tumor at or near the margin is strongly correlated with the risk of local tumor recurrence, so there is a need for a non-invasive, real-time tool to evaluate margin status. This study examined the use of autofluorescence and diffuse reflectance spectroscopy and spectral imaging to evaluate margin status intraoperatively.Materials and Methods: Spectral measurements were taken from the surface of the tissue mass immediately following removal during partial mastectomies and/or from tissues immediately after sectioning by surgical pathology. A total of 145 normal spectra were obtained from 28 patients, and 34 tumor spectra were obtained from 12 patients.Results: After correlation with histopathology, a multivariate statistical algorithm classified the spectra as normal (negative margins) or tumor (positive margins) with 85% sensitivity and 96% specificity. A separate algorithm achieved 100% classification between neo-adjuvant chemotherapy-treated tissues and non-treated tissues. Fluorescence and reflectance-based spectral images were able to demarcate a calcified lesion on the surface of a resected specimen as well.Conclusion: Fluorescence and reflectance spectroscopy could be a valuable tool for examining the superficial margin status of excised breast tumor specimens, particularly in the form of spectral imaging to examine entire margins in a single acquisition. Lasers Surg. Med. 42:1523, 2010. (C) 2010 Wiley-Liss, Inc.