Comparative Susceptibility of Respiratory Viruses to Recombinant Interferons-α2b and -β
Comparative Susceptibility of Respiratory Viruses to Recombinant Interferons-α2b and -β
复制标题
呼吸道病毒对重组干扰素-α2b 和-β 的敏感性比较
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
F. Hayden
中科院分区：
文献类型：
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作者：
S. Sperber;F. Hayden
鼻内重组干扰素-α2b（rIFN-α2b）对鼻病毒引起的自然感冒有保护作用，但对病毒引起的感冒无保护作用。由于rIFN-β serine 17（rIFN-βser）在预防自然鼻病毒感冒方面的活性似乎低于rIFN-α2b，因此我们在两种体外试验中比较了这两种IFN对选定呼吸道病毒的作用。在产量减少试验中，两种IFN对鼻病毒39型和1A型以及冠状病毒229 E具有相当的活性，在IFN浓度为10−11至10−10克蛋白/ml（约2-20 IU/ml）时，其抑制率为90%或更高。rIFN-βser对临床分离的鼻病毒也有相似的活性。在10 - 9克蛋白/毫升的浓度下，两种干扰素都能抑制甲型流感病毒和副流感病毒的生长，但不能抑制腺病毒或呼吸道合胞病毒在细胞培养系统中的生长。因此，rIFN-α2b和rIFN-βser在天然鼻病毒感冒研究中所赋予的不同临床保护作用是可能的。
ABSTRACT Intranasal recombinant interferon-α2b (rIFN-α2b) protects against natural colds due to rhinoviruses, but apparently not against those caused by viruses. Because rIFN-βserine17 (rIFN-βser) appears less active than rIFN-α2b in preventing natural rhinovirus colds, we compared the two IFNs in two in vitro assays against selected respiratory viruses. In a yield reduction assay, both IFNs had comparable activity against rhinovirus types 39 and 1A and coronavirus 229E, which were inhibited by 90% or more at IFN concentrations of 10−11 to 10−10 gram of protein/ml (approximately 2–20 IU/ml). Similar activities were observed with rIFN-βser against rhinoviruses isolated from clinical specimens. At concentrations of 10−9 gram protein/ml, both IFNs inhibited the growth of influenza A and parainfluenza viruses, but not of adenovirus or respiratory syncytial virus in the cell culture systems tested. Thus, the different clinical protection conferred by rIFN-α2b and rIFN-βser in studies of natural rhinovirus cold...
健康成人鼻内施用重组β丝氨酸干扰素的耐受性和功效。
DOI：
10.1093/infdis/158.1.166
发表时间：
1988
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Sperber,SJ;Levine,PA;Innes,DJ;Mills,SE;Hayden,FG
通讯作者：
Hayden,FG