S-Adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archaeal protein related to SAM-dependent halogenases.
S-Adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archaeal protein related to SAM-dependent halogenases.
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DOI：
10.1002/cbic.200800341
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发表时间：
2008-09-22
期刊：
影响因子：
3.2
通讯作者：
Moore, Bradley S.
中科院分区：
文献类型：
--
作者：
Eustaquio, Alessandra S.;Haerle, Johannes;Noel, Joseph P.;Moore, Bradley S.
s -腺苷- l-蛋氨酸（SAM）是一种普遍存在的分子，参与各种生化反应，是仅次于ATP的最常用的酶底物最近报道的参与放线菌次生代谢物生物合成的SAM依赖卤素酶代表了一个新的SAM结合蛋白家族[2,3]，该家族催化l -蛋氨酸（L-met）从SAM与卤化物的亲核置换，形成卤化5 ' -脱氧腺苷（5 ' -XDA）。[4,5]这些酶属于一个超过100种古细菌和细菌蛋白的家族，没有指定的功能（pfam 01887, DUF62）。本文报道了最近测序的海洋细菌Salinispora arenicola CNS-205 （SaDUF62, Sare_1364，基因组加入号NC_ 009953）的DUF62成员在体外没有显著的卤化酶活性，但具有SAM水解酶（腺苷形成）活性。SAM是由ATP和L-met通过SAM合成酶MetK生物合成的。除了作为核酸和蛋白质的甲基供体这一众所周知的重要作用外，SAM的磺基周围碳的亲电特性也使它成为亚甲基、氨基、核糖基和氨基烷基以及5 ' -脱氧腺苷基自由基的来源此外，SAM在细菌[7,8]和植物中作为核糖体开关介导的mek和蛋氨酸生物合成基因反馈调节的分子效应物[b]然而，SAM的监管作用并不局限于met操作子。SAM水平已被证明会影响土壤细菌链霉菌的形态分化和次生代谢物的生物合成——高水平的SAM会导致抗生素过量产生并抑制产孢——至少部分是通过促进调控基因的转录来实现的。[10-12]
S-Adenosyl-L-methionine (SAM) is a ubiquitous molecule that participates in various biochemical reactions, second only to ATP as the most frequently used enzyme substrate.[1] The recently described SAM-dependent halogenases involved in the biosynthesis of secondary metabolites in actinomycetes represent a new family of SAM-binding proteins [2, 3] that catalyze the nucleophilic displacement of L-methionine (L-met) from SAM with halides to form halogenated 5′-deoxyadenosine (5′-XDA).[4, 5] These enzymes belong to a family of over 100 archaeal and bacterial proteins with no assigned function (pfam 01887, DUF62). Here we report that a DUF62 member from the recently sequenced marine bacterium Salinispora arenicola CNS-205 (SaDUF62, Sare_1364, genome accession number NC_ 009953) has no significant halogenase, but instead SAM hydrolase (adenosine-forming) activity in vitro.SAM is biosynthesized from ATP and L-met by SAM synthetase MetK. Besides its well-known and essential role as a methyl donor to nucleic acids and proteins,[6] the electrophilic character of the carbons surrounding the sulfonium group of SAM makes it also a source of methylene, amino, ribosyl, and aminoalkyl groups, as well as 5′-deoxyadenosyl radicals.[1] Furthermore, SAM acts as a molecular effector in the riboswitch-mediated feedback regulation of metK and methionine biosynthesis genes in bacteria [7, 8] and plants.[9] Yet, the regulatory role of SAM is not limited to the met operon. SAM levels have been shown to influence morphological differentiation and secondary-metabolite biosynthesis in the soil bacteria Streptomyces—high levels of SAM cause antibiotic overproduction and inhibit sporulation—at least in part by promoting transcription of regulatory genes.[10–12]
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影响因子：
16.6
作者：
Deng, Hai;Botting, Catherine H.;O'Hagan, David
通讯作者：
O'Hagan, David
DOI：
10.1073/pnas.0700962104
发表时间：
2007-06-19
影响因子：
11.1
作者：
Udwary, Daniel W.;Zeigler, Lisa;Moore, Bradley S.
通讯作者：
Moore, Bradley S.
影响因子：
3.9
作者：
Loenen, WAM
通讯作者：
Loenen, WAM
影响因子：
5.8
作者：
Lambert, C;Léonard, N;Depiereux, E
通讯作者：
Depiereux, E
影响因子：
--
作者：
Kozbial PZ;Mushegian AR
通讯作者：
Mushegian AR