Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s
Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s
复制标题
分支策略对高分支聚β-氨基酯基因转染的影响
DOI：
10.1007/s10118-020-2393-y
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发表时间：
2020
影响因子：
4.3
通讯作者：
Wang Wenxin
中科院分区：
文献类型：
--
作者：
Wang Yao;Wang Chenfei;Li Ming;Zhou Dezhong;Huang Wei;Wang Wenxin
高度支化的聚（β-氨基酯）（HPAEs）已经成为体外和体内最有活力的非病毒基因递送载体之一。然而，不同分支策略对基因转染性能的影响尚未被探索。本文以三丙烯酸酯（B3）和二胺（B4）为支化单体，分别采用“A2 + B3 + C2”和“A2 + B4 + C2”策略合成了一系列HPAEs。结果表明，分支策略在决定HPAE/DNA复合物的生理特性中起着关键作用，从而导致明显不同的细胞活力和转染效率。相比之下，通过“A2 + B3 + C2”分支策略合成的HPAEs比通过“A2 + B4 + C2”策略合成的HPAEs更有利于DNA转染。本研究为基于HPAEs的非病毒DNA递送系统的开发提供了新的思路。
Highly branched poly(β-amino ester)s (HPAEs) have emerged as one type of the most viable non-viral gene delivery vectors, both in vitro and in vivo. However, the effects of different branching strategies on the gene transfection performance have not yet been explored. Here, using triacrylate (B3) and diamine (B4) as the branching monomers, a series of HPAEs were synthesized via the "A2 + B3 + C2" and "A2 + B4 + C2" strategies, respectively. Results show that the branching strategy plays a pivotal role in dictating the physiological properties of the HPAE/DNA polyplexes and thus leads to obviously different cell viability and transfection efficiency. Comparatively, HPAEs synthesized via the "A2 + B3 + C2" branching strategy are more favorable for DNA transfection than that synthesized via the "A2 + B4 + C2" strategy. This study may provide new insights into the development of HPAEs based non-viral DNA delivery system.