Different effects of implanting vascular bundles and sensory nerve tracts on the expression of neuropeptide receptors in tissue-engineered bone in vivo
Different effects of implanting vascular bundles and sensory nerve tracts on the expression of neuropeptide receptors in tissue-engineered bone in vivo
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植入血管束和感觉神经束对体内组织工程骨神经肽受体表达的不同影响
DOI：
10.1088/1748-6041/5/5/055002
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发表时间：
2010-10-01
影响因子：
4
通讯作者：
Pei, Guo-Xian
中科院分区：
文献类型：
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作者：
Chen, Si-Yuan;Qin, Jun-Jun;Pei, Guo-Xian
我们研究了在组织工程骨中植入维管束或感觉神经是否影响降钙素基因相关肽I型受体（CGRP1R）和神经肽Y1受体（NPY1R）的表达。我们将以β-磷酸三钙（β-TCP）为支架材料的成骨诱导骨髓间充质干细胞（BMSCs）与感觉神经束（I组，n = 18）、维管束（II组，n = 18）或单独（III组，n = 18）植入修复兔1.2 cm股骨缺损。4、8、12周时，x线及组织学观察显示，ⅰ、ⅱ组成骨效果优于ⅲ组。在新骨内，实时荧光定量PCR检测的两种神经肽受体mRNA水平在第8周升高后逐渐降低（P < 0.05）。免疫组化法测定的神经肽受体表达在4周时最低（P < 0.05）， II组高于I组（P < 0.05）。在各时间点，I、II组的表达均明显高于III组。我们认为，在组织工程骨中植入维管束可以显著提高CGRP1R和NPY1R的早期表达。相比之下，感觉神经的植入没有表现出与维管束植入相同的显著效果。
We investigated whether implantation of vascular bundles or sensory nerves affected the expression of calcitonin gene-related peptide type I receptor (CGRP1R) and neuropeptide Y1 receptor (NPY1R) in tissue-engineered bone. We implanted osteogenically induced bone marrow mesenchymal stem cells (BMSCs) with β-tricalcium phosphate (β-TCP) as the scaffold material either with sensory nerve tracts (group I, n = 18), vascular bundles (group II, n = 18) or alone (group III, n = 18) to repair a 1.2 cm femur defect in the rabbit. Better osteogenesis was observed by x-ray and histology in groups I and II than in group III at 4, 8 and 12 weeks. Within the new bone, the mRNA levels of the two neuropeptide receptors determined by real-time PCR increased through week 8, and then gradually decreased (P < 0.05). Expression of the neuropeptide receptors determined by immunohistochemistry was lowest at 4 weeks (P < 0.05) and was higher in group II than in group I (P < 0.05). Expression was significantly higher in groups I and II than in group III at all time points. We conclude that implanting vascular bundles into tissue-engineered bone can significantly improve the early expression of CGRP1R and NPY1R. In contrast, implantation of sensory nerves did not show the same dramatic effect as implantation of vascular bundles.