cDNA cloning and gene expression analysis of human myo-inositol 1-phosphate synthase
cDNA cloning and gene expression analysis of human myo-inositol 1-phosphate synthase
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DOI：
10.1016/s0003-9861(03)00388-6
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发表时间：
2003-09-15
影响因子：
3.9
通讯作者：
Shechter, I
中科院分区：
文献类型：
--
作者：
Guan, GM;Dai, PH;Shechter, I
肌醇1-磷酸合酶（myo-Inositol 1-phosphate synthase，IPS）是肌醇生物合成途径中的关键酶。5.5.1.4本研究克隆了人肌醇1-磷酸合酶（myo-inositol 1-phosphate synthase，hIPS）全长cDNA，分析了hIPS mRNA在HepG 2细胞中的组织分布及表达特征。人睾丸、卵巢、心脏、胎盘和胰腺表达相对高水平的hIPS mRNA，而血白细胞、胸腺、骨骼肌和结肠表达低或边缘量的mRNA。在葡萄糖存在下，HepG 2细胞中hIPS mRNA水平增加2- 4倍。hIPS mRNA也被2.5 μ M洛伐他汀上调2- 3倍。这种上调被甲羟戊酸、法尼醇和香叶基香叶醇阻止，表明在hIPS基因表达的调节中G蛋白介导的信号转导机制。在这些细胞中，hIPS mRNA表达被10 mM锂离子抑制50%。无论是5毫米肌醇，也不是三种激素：雌激素，甲状腺激素和胰岛素改变hIPS mRNA在这些细胞中的表达。(C)2003年爱思唯尔公司All rights reserved.
myo-Inositol 1-phosphate synthase (EC 5.5.1.4) (IPS) is a key enzyme in myo-inositol biosynthesis pathway. This study describes the molecular cloning of the full length human myo-inositol 1-phosphate synthase (hIPS) cDNA, tissue distribution of its mRNA and characterizes its gene expression in cultured HepG2 cells. Human testis, ovary, heart, placenta, and pancreas express relatively high level of hIPS mRNA, while blood leukocyte, thymus, skeletal muscle, and colon express low or marginal amount of the mRNA. In the presence of glucose, hIPS mRNA level increases 2- to 4-fold in HepG2 cells. hIPS mRNA is also up-regulated 2- to 3-fold by 2.5 muM lovastain. This up-regulation is prevented by mevalonic acid, farnesol, and geranylgeraniol, suggesting a G-protein mediated signal transduction mechanism in the regulation of hIPS gene expression. hIPS mRNA expression is 50% suppressed by 10 mM lithium ion in these cells. Neither 5 mM myo-inositol nor the three hormones: estrogen, thyroid hormone, and insulin altered hIPS mRNA expression in these cells. (C) 2003 Elsevier Inc. All rights reserved.