Cloning and expression in Escherichia coli of thermophilic Clostridium sp. F1 genes related to cellulose hydrolysis.
Cloning and expression in Escherichia coli of thermophilic Clostridium sp. F1 genes related to cellulose hydrolysis.
复制标题
嗜热梭状芽胞杆菌的克隆和在大肠杆菌中的表达。
DOI：
10.1271/bbb1961.53.905
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Shimada
中科院分区：
文献类型：
--
作者：
K. Sakka;S. Furuse;K. Shimada
梭菌菌株F1（在本实验室分离并鉴定）DNA的11个不同片段已在大肠杆菌中克隆。大肠杆菌，并表现出与纤维素水解相关的酶活性。11 E中的6个。其中2个克隆同时具有纤维二糖水解酶和内切聚糖酶活性，1个克隆同时具有β-葡萄糖苷酶活性，2个克隆同时具有外切葡聚糖酶和木聚糖酶活性。将本研究构建的质粒与C.热纤维素酶基因，得出结论，11个克隆中的7个携带迄今未鉴定的纤维素酶基因，即，4个内切葡聚糖酶基因、1个β-葡萄糖苷酶基因和2个木聚糖酶/外切葡聚糖酶基因，其余4个克隆携带与C.热纤
Eleven distinct fragments of Clostridium sp. stain F1 (which was isolated and identified in this laboratory) DNA have been cloned in E. coli and shown to express enzymatic activities related to cellulose hydrolysis. Six of the 11 E. coli clones showed endoglucanase activity, 2 showed cellobiohy-drolase activity as well as endoglycanase activity, one showed β-glucosidase activity and the 2 others showed exoglucanase activity as well as xylanase activity. On comparing the restriction maps of the plasmids constructed in this study with those of C. thermocellum genes, it was concluded that 7 of the 11 clones carried hitherto unidentified cellulase genes, i.e., 4 endoglucanase genes, 1β-glucosidase gene and 2 xylanase/exoglucanase genes, and that the 4 other clones carried the same genes as those found in C. thermocellum.