A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment
A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment
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DOI：
10.1023/a:1006084305012
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发表时间：
1998-05-01
影响因子：
5.1
通讯作者：
Chun, JY
中科院分区：
文献类型：
--
作者：
Lee, YH;Chun, JY
我们报道了利用聚合酶链式反应(PCR)克隆策略从拟南芥中分离并鉴定了一个新的同源异型盒基因。全长cDNA命名为AthB-12，编码235个氨基酸的蛋白质。它包含保守的DNA结合域和亮氨酸拉链基序，这是同源结构域-亮氨酸拉链转录因子家族的特征。其推导的氨基酸序列在同源结构域(82%)和亮氨酸拉链基序(80%)上与拟南芥Athb-7有80%以上的同源性。然而，在同源结构域和亮氨酸拉链基序之外，同源性明显较低。RNA分析表明，只有一个0.96kb的转录本与Athb-12基因的大小一致。在茎、叶、花和根以及幼苗中都检测到Athb-12的转录本。水分胁迫和外源脱落酸(ABA)处理导致了Athb-12mRNA的积累，与Athb-7的表达类似。然而，Athb-12对ABA的响应时间进程与Athb-7不同，这表明这两个基因对ABA的响应是以不同的方式调控的。综上所述，这些数据表明，Athb-12和Athb-7是参与植物对水分胁迫反应的相关基因家族的成员。
We report the isolation and characterization of a new homeobox gene from Arabidopsis thaliana using a polymerase chain reaction (PCR) cloning strategy. The full-length cDNA, designated Athb-12, encodes a protein of 235 amino acids. It contains the conserved DNA binding domain and the leucine zipper motif, characteristic of the homeodomain-leucine zipper family of transcription factors. The deduced amino acid sequence of Athb-12 shows over 80% identity to the Arabidopsis Athb-7 in the homeodomain (82%) and the leucine zipper motif (80%) of the proteins. However, outside the homeodomain and the leucine zipper motif, the homology is significantly lower. RNA analysis identified only one 0.96 kb transcript consistent with the size of Athb-12 cDNA. The Athb-12 transcript was detected in stem, leaf, flower and root as well as in seedlings. Treatment with water stress and exogenous abscisic acid (ABA) resulted in the accumulation of Athb-12 mRNA, similar to that of Athb-7. However, the time course of the Athb-12 response to ABA differed from that of Athb-7, suggesting that both genes, in response to ABA, are regulated in different manners. Taken together, these data suggest that Athb-12 and Athb-7 are members of a related gene family involved in the plant's response to water stress.