[The protection mechanisms of glycine against liver injury induced by lipopolysaccharides].
[The protection mechanisms of glycine against liver injury induced by lipopolysaccharides].
复制标题
甘氨酸对脂多糖所致肝损伤的保护机制[J].
DOI：
--
复制
发表时间：
2006
影响因子：
--
通讯作者：
J. Gong
中科院分区：
文献类型：
--
作者：
H. You;Qiang Wang;Xu;Xian;Zuo;J. Gong
目的
探讨甘氨酸（Gly）对脂多糖（LPS）诱导的肝损伤的保护作用机制。
方法
将BABL/c小鼠随机分为LPS组和Gly组，LPS组腹腔注射10 mg/kg LPS，Gly组在给予相同剂量的LPS前用含5% Gly的饮食预处理3 d。检查小鼠肝脏的组织病理学变化。使用ELISA分析测量血浆中的TNF α和白细胞介素-10（IL-10）水平。RT-PCR检测肝组织TNF α、IL-10和Toll样受体4（TLR 4）mRNA的表达。免疫组化法检测肝组织TLR 4蛋白表达。
结果
与LPS组动物相比，Gly组动物的存活率提高，且LPS诱导的肝组织病理变化减轻。TNF α水平[（1，852.80 +/-126.64）pg/ml vs（708.83 ± 51.29）pg/ml，P<0.05]，TNF α的表达肝组织中TLR 4（A0.97 +/-0.12vs.0.53 +/-0.11，P <0.05）mRNA表达降低。血浆IL-10水平[（344.09+/-31.70）pg/ml vs（418.64+/-38.86）pg/ml，P<0.05]显著升高，达峰时间左移。
结论
Gly预处理可减轻LPS诱导的小鼠肝损伤，其机制可能与其下调TLR 4的表达，上调IL-10的产生有关。
OBJECTIVE
To explore the protective mechanisms of glycine (Gly) on lipopolysaccharides (LPS) induced liver injury.
METHODS
BABL/c mice were randomly divided into a LPS group, in which the animals were intraperitoneally injected with 10 mg/kg LPS, and a Gly group, in which the mice were pretreated with a 5% Gly-containing diet for 3 days before receiving the same dose of LPS. The livers of the mice were examined for histopathological changes. The TNF alpha and interleukin-10 (IL-10) levels in the blood plasma were measured using ELISA analysis. The mRNA expression of TNF alpha, IL-10 and Toll-like receptor 4 (TLR4) in hepatic tissues were detected using RT-PCR analysis. Protein expression of TLR4 in livers was detected using immunohistochemistry.
RESULTS
The Gly group mice had an improved survival rate and attenuated LPS-induced pathological changes in the liver tissues in comparison with those of the LPS group animals. The TNF alpha levels [(1,852.80+/-126.64) pg/ml vs (708.83+/-51.29) pg/ml, P<0.05] in plasma, as well as the expression of TNF alpha (A 1.59+/-0.14 vs. 0.91+/-0.11, P<0.05) and TLR4 (A 0.97+/-0.12 vs. 0.53+/-0.11, P<0.05) mRNA in liver tissues were decreased. However, the levels of plasma interleukin-10 [(344.09+/-31.70) pg/ml vs (418.64+/-38.86) pg/ml, P<0.05] were significantly increased and the peaking time left, shifted.
CONCLUSIONS
Gly pretreatment could attenuate LPS -induced liver injury in mice, which may be associated with its role in down-regulating TLR4 expression and up-regulating IL-10 production.