The Salmonella enterica serovar Typhimurium QseB response regulator negatively regulates bacterial motility and swine colonization in the absence of the QseC sensor kinase
The Salmonella enterica serovar Typhimurium QseB response regulator negatively regulates bacterial motility and swine colonization in the absence of the QseC sensor kinase
复制标题
DOI：
10.1016/j.micpath.2010.03.005
复制
发表时间：
2010-06-01
影响因子：
3.8
通讯作者：
Brunelle, Brian W.
中科院分区：
文献类型：
--
作者：
Bearson, Bradley L.;Bearson, Shawn M. D.;Brunelle, Brian W.
鼠伤寒沙门氏菌（S.鼠伤寒沙门氏菌）通过增加细菌生长和增强运动性对儿茶酚胺、去甲肾上腺素作出反应。在这项研究中，铁与或不含铁载体，ferrioxamine E也增强了细菌的运动。铁增强运动是生长速度依赖性的，而去甲肾上腺素增强运动是生长速度无关的。外膜儿茶酚受体，IroN，FepA和CirA（所需的去甲肾上腺素增强生长）不需要去甲肾上腺素增强的运动，也不是ExbD的能量转导TonB-ExbB-ExbD铁-铁载体摄取系统。对QseBC双组分系统的检查显示，qseB和qseBC突变体具有与野生型S相似的运动表型。而qseC突变体的运动能力显著降低（P < 0.01）。QseBC系统的每个突变体，以及qseE和pmrA的突变体，对去甲肾上腺素的反应增加了运动性，这表明其他基因参与了去甲肾上腺素增强的S.鼠伤寒。在猪宿主中，qseBC突变体的粪便脱落与野生型S相似。而qseC突变体的粪便脱落显著减少（P < 0.01）。我们的数据表明，在qseC突变体中，QseB反应调节剂降低了运动性和猪定植; qseBC操纵子的失活恢复了这些细菌表型，将QseB归类为细菌运动性和猪定植的负调节剂。爱思唯尔有限公司出版
Salmonella enterica serovar Typhimurium (S. Typhimurium) responds to the catecholamine, norepinephrine by increasing bacterial growth and enhancing motility. In this study, iron with or without the siderophore, ferrioxamine E also enhanced bacterial motility. Iron-enhanced motility was growth-rate dependent, while norepinephrine-enhanced motility was growth-rate independent. The outer membrane catecholate receptors, IroN, FepA and CirA (required for norepinephrine-enhanced growth) were not required for norepinephrine-enhanced motility, nor was ExbD of the energy-transducing TonB-ExbB-ExbD ferri-siderophore uptake system. Examination of the QseBC two-component system revealed that qseB and qseBC mutants have motility phenotypes similar to wild-type S. Typhimurium, while motility of the qseC mutant was significantly decreased (P < 0.01). Each mutant of the QseBC system, as well as mutants of qseE and pmrA, responded to norepinephrine with increased motility, suggesting that other genes are involved in norepinephrine-enhanced motility of S. Typhimurium. In the swine host, fecal shedding of the qseBC mutant was similar to wild-type S. Typhimurium, whereas fecal shedding of the qseC mutant was significantly decreased (P < 0.01). Our data indicate that, in a qseC mutant, the QseB response regulator decreases motility and swine colonization; inactivation of the qseBC operon restores these bacterial phenotypes, classifying QseB as a negative regulator of bacterial motility and swine colonization. Published by Elsevier Ltd.