CIS-TRANS RECOGNITION AND SUBUNIT-SPECIFIC DEGRADATION OF SHORT-LIVED PROTEINS
CIS-TRANS RECOGNITION AND SUBUNIT-SPECIFIC DEGRADATION OF SHORT-LIVED PROTEINS
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DOI：
10.1038/346287a0
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发表时间：
1990-07-19
期刊：
影响因子：
64.8
通讯作者：
VARSHAVSKY, A
中科院分区：
文献类型：
--
作者：
JOHNSON, ES;GONDA, DK;VARSHAVSKY, A
N-末端规则是一种将蛋白质的代谢稳定性与其氨基末端残基的身份联系起来的密码1，它是普遍的，因为不同版本的N-末端规则在哺乳动物2 -5、酵母1，6，7和细菌（未发表的数据）中起作用。基于N端规则的降解信号包括一个不稳定的氨基末端残基和一个特定的内部赖氨酸残基1，6，7。我们现在表明，在多亚基蛋白质中，这两个决定簇可以位于不同的亚基上，并且仍然靶向蛋白质进行破坏。此外，在这种情况下（反式识别），只有携带赖氨酸决定簇的亚基实际上被降解。因此，寡聚蛋白质可以包含短寿命和长寿命亚基。这些见解具有功能和实际意义。
THE N-end rule, a code that relates the metabolic stability of a protein to the identity of its ammo-terminal residue1, is universal in that different versions of the N-end rule operate in mammals2–5, yeast1,6,7and bacteria (unpublished data). The N-end rule-based degradation signal comprises a destabilizing amino-terminal residue and a specific internal lysine residue1,6,7. We now show that, in a multisubunit protein, these two determinants can be located on different subunits and still target the protein for destruction. Moreover, in this case (transrecognition) only the subunit that bears the lysine determinant is actually degraded. Thus an oligomeric protein can contain both short-lived and long-lived subunits. These insights have functional and practical implications.