Baseline sensitivity of natural population and resistance risk of Peronophythora litchii to four novel QoI fungicides
Baseline sensitivity of natural population and resistance risk of Peronophythora litchii to four novel QoI fungicides
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DOI：
10.1007/s10658-016-0893-y
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发表时间：
2016-04
影响因子：
1.8
通讯作者：
Yuxin Zhou;Lei Chen;Jian Hu;Pengfei Liu;Yu Zhang;Q. Meng;Bin Li;N. Si;Changling Liu;Xili Liu
中科院分区：
文献类型：
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作者：
Yuxin Zhou;Lei Chen;Jian Hu;Pengfei Liu;Yu Zhang;Q. Meng;Bin Li;N. Si;Changling Liu;Xili Liu
采用离体菌丝生长法测定了68个荔枝霜疫霉（Peronophythora litchii）田间菌株对4种新的QoI杀菌剂（SYP-1620、SYP-2815、ZJ 0712和烯肟菌酯）的敏感性。SYP-2815、ZJ 0712、SYP-1620和enestroburin的EC 50值（μg/mL）的范围和平均值分别为0.047-0.288和0.138、0.056-0.992和0.148、0.430-7.529和2.720、0.180-1.772和0.810;分布近似为单峰和偏态。通过将田间分离物暴露于杀菌剂修正的琼脂和紫外线照射，获得了对三种新型QoI杀菌剂和嘧菌酯（作为已知QoI杀菌剂）具有抗性的8个突变体，而没有获得对烯肟菌酯具有抗性的突变体。在实验室测定中，所有8个突变体都具有高而稳定的抗性，并且大多数与敏感菌株一样适合或更适合。5种杀菌剂之间存在交互抗性。P. 4种新杀菌剂对荔枝疫霉菌的抗性不一致，如果在田间频繁使用这些杀菌剂，荔枝疫霉菌对SYP-2815、ZJ 0712和SYP-1620产生抗性的可能性很大。
Sixty-eight field isolates ofPeronophythora litchii, the causal agent of litchi downy blight, were examined for sensitivity to four novel QoI fungicides (SYP-1620, SYP-2815, ZJ0712 and enestroburin) in vitro with a mycelial growth assay. Ranges and means of EC50values (in μg/mL) were 0.047–0.288 and 0.138 for SYP-2815, 0.056–0.992 and 0.148 for ZJ0712, 0.430–7.529 and 2.720 for SYP-1620, and 0.180–1.772 and 0.810 for enestroburin; the distributions were approximately unimodal and skewed. Eight mutants resistant to the three novel QoI fungicides and azoxystrobin (as a known QoI fungicide) were obtained by exposing field isolates to fungicide-amended agar and to ultraviolet irradiation, while mutants resistant to enestroburin were not obtained. In laboratory assays, all eight mutants had a high and stable resistance, and most were as fit or more fit than sensitive isolates. There was cross-resistance among the five QoI fungicides. The resistance risk ofP. litchiito the four novel QoI fungicides was inconsistent, and the probability thatP.litchiiwill develop resistance to SYP-2815, ZJ0712, and SYP-1620 may be high if these fungicides are frequently applied in the field.