Effect of recombinant human granulocyte colony-stimulating factor (rh G-CSF) on murine resistance against Listeria monocytogenes.
Effect of recombinant human granulocyte colony-stimulating factor (rh G-CSF) on murine resistance against Listeria monocytogenes.
复制标题
重组人粒细胞集落刺激因子（rh G-CSF）对小鼠单核细胞增生李斯特菌耐药性的影响。
作者：
B. Serushago;Y. Yoshikai;T. Handa;M. Mitsuyama;K. Muramori;K. Nomoto
重组人粒细胞集落刺激因子（rhG-CSF）增强小鼠对单核细胞增多性李斯特菌（LM）的抵抗力，通过存活率和细菌生长测定。用rhG-CSF每天两次预处理5天的小鼠比未处理的动物对LM的攻击存活得更好。治疗组小鼠腹腔和脾脏细菌数均低于对照组。Rh G-CSF主要使血液和脾脏中的多形核白细胞（PMN）增多。接种LM后，小鼠PC和脾脏中有大量的PMN和单核-巨噬细胞聚集。此外，在体内与rhG-CSF引发的PMN释放更多的超氧阴离子时，刺激佛波醇肉豆蔻酸酯乙酸酯。PC和脾脏中细菌生长的抑制可归因于感染部位吞噬细胞的积聚和氧化代谢的增加。这些结果进一步证明了G-CSF和中性粒细胞作为靶细胞对宿主防御胞内细菌的重要贡献。
Recombinant human granulocyte colony-stimulating factor (rh G-CSF) enhanced resistance of mice against Listeria monocytogenes (LM) as determined by survival and bacterial growth. Mice pretreated with rh G-CSF twice daily for 5 days survived better than untreated animals to the challenge with LM. Number of bacteria in peritoneal cavity (PC) and spleen was lower in treated mice than that in the control group. Rh G-CSF increased mainly polymorphonuclear cells (PMN) in blood and spleen. After LM inoculation, a larger number of PMN and monocyte-macrophages accumulated in PC and spleen of tested mice. In addition, PMN primed in vivo with rh G-CSF released more superoxide anions when stimulated with phorbol myristate acetate. The inhibition of bacterial growth in PC and spleen could be ascribed to the accumulation of phagocytic cells at the infection sites and the increased oxidative metabolism. The results provided further evidence of the important contribution of G-CSF and neutrophils, as target cells, to the host defence against the intracellular bacteria.