Expression of survivin, PTEN and p27 in normal, hyperplastic, and carcinomatous endometrium
Expression of survivin, PTEN and p27 in normal, hyperplastic, and carcinomatous endometrium
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DOI：
10.1111/j.1525-1438.2006.00541.x
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发表时间：
2006-05-01
影响因子：
4.8
通讯作者：
Demirhan, B.
中科院分区：
文献类型：
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作者：
Erkanli, S.;Kayaselcuk, F.;Demirhan, B.
我们的目的是研究生存素和p27蛋白的表达是否参与子宫内膜样癌的发展，沿着这些蛋白与野生型PTEN缺失之间是否存在任何相关性，野生型PTEN缺失在高达80%的子宫内膜癌中发现。我们还研究了它们与子宫内膜癌经典预后因素和生存率的相关性。据我们所知，这是第一次研究生存素在子宫内膜增生症和子宫内膜样腺癌中的表达，沿着。在病理学档案中选择29例子宫内膜样腺癌、38例子宫内膜增生和10例增生性子宫内膜组织样本进行免疫组化分析。如果> 50%，则细胞染色评分为+2，如果<50%，则评分为+1，如果没有染色阳性，则评分为阴性。Survivin的表达从增殖期到增生期再到癌均呈上升趋势。在子宫内膜增生症和子宫内膜癌中，PTEN和p27的表达均低于增生期子宫内膜。差异有统计学意义（P < 0.05）。PTEN与p27呈正相关（P < 0.05），而与Survivin均无相关性。这些基因均与子宫内膜样腺癌的分级和肌层浸润等经典预后因素无关。然而，PTEN阳性病例的平均生存期在统计学上显著更高（46.6个月对16.4个月）（P < 0.05）。Survivin的过度表达可能与PTEN、p27活性的缺失或降低一起沿着在类胶质腺癌发生发展中起重要作用。然而，Survivin在类腺癌的发生发展中似乎以不同于PTEN或p27的方式发挥其作用。这些发现生存素的作用在类腺癌应得到证实，并通过生存素的途径，在类腺癌的行为进行了进一步研究，更大的样本量。
We aimed to investigate if expressions of survivin and p27 proteins are involved in the development of endometrioid carcinoma, along with whether there are any correlations between these proteins and loss of wild-type PTEN that is found in up to 80% of endometrial carcinomas. We also studied their correlations with classical prognostic factors and survival in endometrial carcinoma. To our knowledge, this is the first time survivin expression is investigated in endometrial hyperplasia along with endometrioid adenocarcinoma. For immunohistochemical analysis, 29 endometrioid adenocarcinoma, 38 endometrial hyperplasia, and 10 proliferative endometrium tissue samples were selected in the pathology archives. Staining of cells was scored as +2 if > 50%, +1 if < 50%, and negative if none were stained positive. Survivin expression increased from proliferative to hyperplasia to carcinoma cases. PTEN and p27 expressions decreased in hyperplasia and carcinoma cases with respect to proliferative endometrium. All these differences were statistically significant (P < 0.05). PTEN positively correlated to p27 (P < 0.05); however, neither was correlated with survivin. None of these genes were correlated with classical prognostic factors such as grade and myometrial invasion in endometrioid adenocarcinoma. However, mean survival was statistically significantly higher in PTEN-positive cases (46.6 vs 16.4 months) (P < 0.05). Survivin overexpression might be one of the important mechanisms in the development of endometrioid adenocarcinoma along with lost or decreased activity of PTEN and p27. However, survivin seems to exert its role in ways different from those of PTEN or p27 in the development of endometrioid adenocarcinoma. These findings on the role of survivin in endometrioid adenocarcinoma should be confirmed and the pathways through which survivin acts in endometrioid adenocarcinoma studied further with a larger sample size.