Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites.
Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites.
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从患病和健康牙周部位分离出的黑色厌氧菌的特征。
DOI：
10.1111/j.1600-0765.1995.tb02129.x
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发表时间：
1995
影响因子：
3.5
通讯作者：
T. F. Walsh
中科院分区：
文献类型：
--
作者：
R. Teanpaisan;C. Douglas;T. F. Walsh
中间普雷沃氏菌（Prevotella intermedia）最近被重新定义，并提出了一个新种--变黑普雷沃氏菌（Prevotella nigrescens）。然而，关于这些新物种在牙周健康或疾病中的发病率的数据很少。通过血清分型、SDS-PAGE和生理学试验对从患病和健康龈下部位分离的黑色厌氧菌进行鉴定。在成人牙周炎受试者中，64%的活动部位，35.7%的非活动部位和38.5%的健康部位产生黑色厌氧菌。其中，牙龈卟啉单胞菌被发现在11%的活动和5%的健康部位的患病患者，中间普雷沃菌在15.5%的活动和20.5%的健康部位，变黑普雷沃菌在37.7%的活动和11.5%的健康部位和齿垢普雷沃菌在3%的活动和1%的健康部位。在健康受试者中，50%的部位产生黑色厌氧菌。在健康受试者中未发现牙龈卟啉单胞菌，但在18%的部位发现中间卟啉单胞菌，在31%的部位发现变黑卟啉单胞菌。SDS-PAGE是一种常规鉴别中间青霉和黑青霉的有效方法，并根据带型将后者分为两个亚型。在任何给定的个体中仅发现一种变黑假单胞菌亚型，并且以ATCC 25261为代表的一种类型在深口袋中更常见。然而，总的来说，无论是P. nigrescens和P. intermedia作为物种，只是经常发现在健康的网站，患病的网站。因此，与牙龈卟啉单胞菌相比，这些菌种似乎是常见的细菌，但它们可能作为机会致病菌。
Prevotella intermedia has recently been re-defined and a new species, Prevotella nigrescens has been proposed. However, there is little data available on the incidence of these new species in periodontal health or disease. Black-pigmented anaerobes isolated from diseased and healthy subgingival sites were identified by serotyping, SDS-PAGE and physiological tests. In adult periodontitis subjects, 64% of active sites, 35.7% of inactive sites and 38.5% of healthy sites yielded black-pigmented anaerobes. Of these, Porphyromonas gingivalis was found in 11% of active and 5% of healthy sites in diseased patients, Prevotella intermedia in 15.5% of active and 20.5% of healthy sites, Prevotella nigrescens in 37.7% of active and 11.5% of healthy sites and Prevotella denticola in 3% of active and 1% of healthy sites. In healthy subjects, 50% of sites yielded black-pigmented anaerobes. P. gingivalis was not found in healthy subjects but P. intermedia was found in 18% and P. nigrescens in 31% of sites. SDS-PAGE proved to be a useful method for routinely differentiating P. intermedia and P. nigrescens and two sub-types of the latter species were detected on the basis of band pattern. Only one P. nigrescens sub-type was found in any given individual and one type, typified by ATCC 25261, was more commonly found in deep pockets. However, overall both P. nigrescens and P. intermedia as species were just as frequently found at healthy sites as diseased sites. Thus, these species, in contrast to P. gingivalis, appear to be common commensals but they may act as opportunistic pathogens.
成人牙周炎治疗后某些龈下细菌与牙周袋深度和牙周附着获得或丧失的关系。
影响因子：
6.7
作者：
Slots,J;Emrich,LJ;Genco,RJ;Rosling,BG
通讯作者：
Rosling,BG
某些龈下微生物物种在选定牙周条件下的分布。
影响因子：
3.5
作者：
Savitt,ED;Socransky,SS
通讯作者：
Socransky,SS
影响因子：
6.7
作者：
DZINK, JL;SOCRANSKY, SS;HAFFAJEE, AD
通讯作者：
HAFFAJEE, AD