On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays.
On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays.
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DOI：
10.1038/ncomms4741
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发表时间：
2014-05-06
影响因子：
16.6
通讯作者：
Jin, Dayong
中科院分区：
文献类型：
--
作者：
Lu, Yiqing;Lu, Jie;Zhao, Jiangbo;Cusido, Janet;Raymo, Francisco M.;Yuan, Jingli;Yang, Sean;Leif, Robert C.;Huo, Yujing;Piper, James A.;Robinson, J. Paul;Goldys, Ewa M.;Jin, Dayong
最近，通过调节被称为“τ - 点”的上转换纳米粒子的发光寿命，已经证明了光时域编码具有显著的复用能力。它提供了从微秒到毫秒的大范围动态寿命，这使得创建大量的纳米标签/微载体库成为可能。然而，需要一种可靠的方法来从相对缓慢衰减的信号中快速且准确地测量发光寿命。在此我们展示了一种适用于微秒区域的快速算法，其精度接近理论极限，并与快速扫描细胞术技术兼容。我们利用这种方法将光时域复用进一步扩展到下转换发光，使用发光微球，其中通过发光共振能量转移来调节寿命。我们在快速扫描细胞术中演示了对这些微球的实时区分，并将它们应用于病原体DNA链的多重探测。我们的结果表明，可调谐的发光寿命在高通量分析科学中具有相当大的潜力。
准确确定缓慢衰减信号的发光寿命可能具有挑战性。在这里，作者报告了一种拟合算法，并随后通过实验展示了一种在微秒区域快速测量发光寿命的方法。
Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called ‘τ-Dots’. It provides a large dynamic range of lifetimes from microseconds to milliseconds, which allows creating large libraries of nanotags/microcarriers. However, a robust approach is required to rapidly and accurately measure the luminescence lifetimes from the relatively slow-decaying signals. Here we show a fast algorithm suitable for the microsecond region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique. We exploit this approach to further extend optical time-domain multiplexing to the downconversion luminescence, using luminescence microspheres wherein lifetimes are tuned through luminescence resonance energy transfer. We demonstrate real-time discrimination of these microspheres in the rapid scanning cytometry, and apply them to the multiplexed probing of pathogen DNA strands. Our results indicate that tunable luminescence lifetimes have considerable potential in high-throughput analytical sciences. Accurately determining luminescence lifetimes for slow-decaying signals can be challenging. Here, the authors report a fitting algorithm and subsequently experimentally show a method for the rapid measurement of luminescence lifetimes in the microsecond region.
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DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
3.7
作者：
Gahlaut, Nivriti;Miller, Lawrence W.
通讯作者：
Miller, Lawrence W.
DOI：
10.1073/pnas.95.4.1556
发表时间：
1998-02-17
影响因子：
11.1
作者：
Eggeling, C;Fries, JR;Seidel, CAM
通讯作者：
Seidel, CAM
影响因子：
15
作者：
Kim, Sung Hoon;Gunther, Jillian R.;Katzenellenbogen, John A.
通讯作者：
Katzenellenbogen, John A.
影响因子：
3.7
作者：
Cui, HH;Valdez, JG;Crissman, HA
通讯作者：
Crissman, HA