Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops.
Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops.
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DOI：
10.1098/rspb.2015.0964
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发表时间：
2015-07-22
期刊：
影响因子：
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通讯作者：
Shinozaki K
中科院分区：
文献类型：
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作者：
Onda Y;Hashimoto K;Yoshida T;Sakurai T;Sawada Y;Hirai MY;Toyooka K;Mochida K;Shinozaki K
二穗短柄草是研究温带禾本科植物生物学现象的一种新兴模式植物。生长尺度的研究对于分析整个生命周期中分子因子的时空变化至关重要。用于基于B中形态学的敏感和稳健分期。在二穗型小麦中，我们证明了BBCH（Biologische Bundesanstalt，Bundessortenamt and Chemical industry）规模的实用性，其与常规用于小麦族作物的Zadoks规模相当。我们比较了B的时间进程。二穗小麦Bd 21和Bd 3 -1，以及中国春小麦的进展。生长阶段的比较说明了形态的相似性和差异的生活史事件的时间。此外，我们使用广泛针对性的代谢组学分析比较了不同生长阶段和不同胁迫条件下的代谢物积累模式。代谢特征分析确定了取决于生物体、生长阶段和/或胁迫条件的化学特性的共性和特异性。大多数代谢物在B中的蓄积量相当。二穗小麦这种定性相似性表明了B的优越性。二穗草作为小麦族作物的模式。B的生长规模。distachyon应该提供一个概念框架的比较分析和知识整合之间的模式草和作物Pooideae亚科。
Brachypodium distachyon is an emerging model plant for studying biological phenomena in temperate grasses. Study of the growth scale is essential to analyse spatio-temporal changes in molecular factors throughout the life cycle. For sensitive and robust staging based on morphology in B. distachyon, we demonstrated the utility of the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) scale, which is comparable to the Zadoks scale conventionally used for Triticeae crops. We compared the chronological progression of B. distachyon accessions Bd21 and Bd3-1, in addition to the progression of Chinese Spring wheat. The comparison of growth stages illustrates the morphological similarities and differences in the timing of life cycle events. Furthermore, we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis. Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions. Most metabolites accumulated equivalently in B. distachyon and wheat. This qualitative similarity indicated the superiority of B. distachyon as a model for Triticeae crops. The growth scale of B. distachyon should provide a conceptual framework for comparative analysis and for knowledge integration between this model grass and crops in the Pooideae subfamily.