Thermal damage threshold of neurons during infrared stimulation
Thermal damage threshold of neurons during infrared stimulation
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DOI：
10.1364/boe.383165
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发表时间：
2020-04-01
影响因子：
3.4
通讯作者：
Stoddart, Paul R.
中科院分区：
文献类型：
--
作者：
Brown, William G. A.;Needham, Karina;Stoddart, Paul R.
在红外神经刺激（INS）中，激光诱发的热瞬变用于在神经元中产生小的去极化电流。激光照射对靶神经元造成中度热损伤风险。事实上，神经刺激的外源性方法经常将靶神经元置于应激的非生理条件下，这会阻碍正常的神经元功能并加速细胞死亡。因此，量化神经元损伤的依赖性概率对于确定INS和其他治疗和假体使用干预的安全操作限值至关重要。使用膜片钳记录在孤立的螺旋神经节神经元，我们描述了一种方法，用于确定剂量依赖性的损伤概率的个别神经元的急性和累积的红外线暴露参数的基础上，在注射电流的变化。结果确定了约60 ℃的局部热损伤阈值，这与之前的文献一致，并支持INS期间的损伤是一种纯粹的热现象的说法。原则上，这种方法可以应用于任何潜在的有害刺激，允许计算范围广泛的剂量依赖性神经损伤概率。与组织学分析不同，该技术非常适合于量化逐渐的神经元损伤，并且不需要临界阈值行为。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
In infrared neural stimulation (INS), laser-evoked thermal transients are used to generate small depolarising currents in neurons. The laser exposure poses a moderate risk of thermal damage to the target neuron. Indeed, exogenous methods of neural stimulation often place the target neurons under stressful non-physiological conditions, which can hinder ordinary neuronal function and hasten cell death. Therefore, quantifying the exposure-dependent probability of neuronal damage is essential for identifying safe operating limits of INS and other interventions for therapeutic and prosthetic use. Using patch-clamp recordings in isolated spiral ganglion neurons, we describe a method for determining the dose-dependent damage probabilities of individual neurons in response to both acute and cumulative infrared exposure parameters based on changes in injection current. The results identify a local thermal damage threshold at approximately 60 degrees C, which is in keeping with previous literature and supports the claim that damage during INS is a purely thermal phenomenon. In principle this method can be applied to any potentially injurious stimuli, allowing for the calculation of a wide range of dose-dependent neural damage probabilities. Unlike histological analyses, the technique is well-suited to quantifying gradual neuronal damage, and critical threshold behaviour is not required. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement