Semi-continuous xylose-to-xylitol bioconversion by Ca-alginate entrapped yeast cells in a stirred tank reactor
Semi-continuous xylose-to-xylitol bioconversion by Ca-alginate entrapped yeast cells in a stirred tank reactor
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DOI：
10.1007/s00449-007-0187-z
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发表时间：
2008-08-01
影响因子：
3.8
通讯作者：
Silva, Silvio S.
中科院分区：
文献类型：
--
作者：
Carvalho, Walter;Canilha, Larissa;Silva, Silvio S.
将季也蒙念珠菌FTI 20037细胞包埋在藻酸钙珠中，并在搅拌釜反应器中连续五批用于木糖至木糖醇的生物转化。以甘蔗渣半纤维素水解液为发酵培养基。藻酸钙珠粒的平均体积在进行五次生物转化循环所花费的600小时后减少约30%，从而证明在反应器容器中占主导地位的条件下的物理不稳定性。尽管如此，沿着重复批次观察到几乎稳定的生物转化率和产率。平均每批产量为51.6克·升（-1），生产率为0.43克·升（-1）·小时（-1），产率为0.71克·克（-1），变异系数小于10%。
Candida guilliermondii FTI 20037 cells were entrapped in Ca-alginate beads and used for xylose-to-xylitol bioconversions during five successive batches in a stirred tank reactor. Supplemented sugarcane bagasse hemicellulosic hydrolysate was used as the fermentation medium. The average volume of the Ca-alginate beads was reduced by about 30% after the 600 h taken to perform the five bioconversion cycles, thus demonstrating physical instability under the conditions prevailing in the reactor vessel. In spite of this, almost steady bioconversion rates and yields were observed along the repeated batches. In average values, a production of 51.6 g l(-1), a productivity of 0.43 g l(-1) h(-1) and a yield of 0.71 g g(-1) were attained in each batch, variation coefficients being smaller than 10%.