Effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa
Effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa
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DOI：
10.1262/jrd.18037
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发表时间：
2006-12-01
影响因子：
1.8
通讯作者：
Tsujii, Hirotada
中科院分区：
文献类型：
--
作者：
Miah, A. G.;Tareq, K. M. A.;Tsujii, Hirotada
松弛素是一种在精浆中发现的多肽激素，在某些物种中对精子活力有生理影响。松弛素对猪精子顶体反应和葡萄糖利用的影响尚未见报道。为了研究松弛素对精子活力、顶体反应和标记葡萄糖掺入和氧化的影响，本研究对猪精子进行洗涤和预孵育，然后在mTALP中分别与0、20和40 ng/ml松弛素孵育(0~6h)。结果表明，在孵育1~4h内，加入松弛素能显著提高精子活力(P<；0.05)。20或40 ng/ml松弛素组精子顶体反应率显著高于对照组(P<0.05)。与对照精子相比，与松弛素孵育的精子的掺入速率和葡萄糖的氧化程度也更高(P<；0.05)。C-14-葡萄糖的掺入速率和氧化速率在孵育4h后随孵育时间的延长而降低，与顶体反应呈正相关。综上所述，本研究表明松弛素不仅能促进猪精子的运动，还能促进顶体反应和葡萄糖的利用。
Relaxin is a peptide hormone found in seminal plasma that has a physiological influence on sperm motility in some species. There are no reports on the effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. In this study, to investigate the effects of relaxin on sperm motility, acrosome reaction, and incorporation and oxidation of labeled glucose, boar spermatozoa were washed and preincubated for swim-up and then incubated (0-6 h) with 0, 20, or 40 ng/ml relaxin in mTALP medium. The results indicated that the addition of relaxin stimulated sperm motility significantly (P < 0.05) during 1-4 h of incubation. The percentage of acrosome-reacted live spermatozoa was higher (P < 0.05) when the spermatozoa were treated with 20 or 40 ng/ml relaxin. The rate of incorporation, and oxidation of glucose were also greater (P < 0.05) in the spermatozoa incubated with relaxin compared to the control spermatozoa. The rate of incorporation and oxidation of C-14-glucose were increased in correlation with acrosome reaction up to 4 h of incubation and then decreased in line with the increasing incubation period. In conclusion, the present study demonstrates that relaxin accelerates not only motility but also the acrosome reaction and utilization of glucose in boar spermatozoa.